CAJ | 학술논문

目的：探讨阿托伐他汀对糖尿病合并冠心病患者外周血单个核细胞NF－κB活性的影响。方法150例患者根据CAG结果及临床病史分为对照组（A组）30例、冠心病组（B组）60例、糖尿病合并冠心病组（C组）60例，再将B组和C组按数字表法随机分为他汀10 mg亚组和40mg 亚组各30例，采用酶联免疫吸附法、乳胶增强透射免疫比浊法和流式细胞仪方法检测各组患者治疗前后外周血白细胞介素－6（IL－6）、高敏C反应蛋白（hs－CRP）水平和单个核细胞NF－κB活性。结果（1）与A组比较，B组和C组外周血单个核细胞NF－κB及IL－6、hs－CRP水平活性均不同程度升高，他汀治疗后NF－κB活性及IL－6、hs－CRP水平下降，且他汀40 mg亚组较他汀10 mg亚组下降更显著（P ＜0．05或P ＜0．01）；（2）患者单个核细胞NF－κB活性与外周血IL－6、hs－CRP水平呈正相关（ r ＝0．550，0．675， P ＜0．01）。结论糖尿病合并冠心病患者外周血单个核细胞NF－κB明显激活，高剂量他汀可显著抑制体内炎性反应。
목적：탐토아탁벌타정대당뇨병합병관심병환자외주혈단개핵세포NF－κB활성적영향。방법150례환자근거CAG결과급림상병사분위대조조（A조）30례、관심병조（B조）60례、당뇨병합병관심병조（C조）60례，재장B조화C조안수자표법수궤분위타정10 mg아조화40mg 아조각30례，채용매련면역흡부법、유효증강투사면역비탁법화류식세포의방법검측각조환자치료전후외주혈백세포개소－6（IL－6）、고민C반응단백（hs－CRP）수평화단개핵세포NF－κB활성。결과（1）여A조비교，B조화C조외주혈단개핵세포NF－κB급IL－6、hs－CRP수평활성균불동정도승고，타정치료후NF－κB활성급IL－6、hs－CRP수평하강，차타정40 mg아조교타정10 mg아조하강경현저（P ＜0．05혹P ＜0．01）；（2）환자단개핵세포NF－κB활성여외주혈IL－6、hs－CRP수평정정상관（ r ＝0．550，0．675， P ＜0．01）。결론당뇨병합병관심병환자외주혈단개핵세포NF－κB명현격활，고제량타정가현저억제체내염성반응。
Objective To investigate the effect of atorvastain on nuclear factor kappa B activity in patients with dia-betes mellitus (DM) combined coronary heart disease (CHD).Methods There were one hundred and fifty patients, thirty of them were divided into control group ( group A) , sixty of them were divided into coronary heart disease group ( group B) , and the last sixty of them were divided into diabetes mellitus combined coronary heart disease group (group C).The patients of group C and group B were randomly divided into two subgroups:10 mg of atorvastatin group and 40 mg of atorvastatin group. The activity of nuclear factor kappa B and level of IL-6, hs-CRP were tested by flow cytometry method, enzyme linked immu-nosorbent method and latex enhanced transmission cells immune turbidimetric method.Results ( 1 ) Compared with group A, the level of IL-6, hs-CRP and activity of NF-κB in group B and group C were increased.After treated with atorvastain,the level of IL-6,hs-CRP and activity of NF-κB were decreased,and 40mg atorvastain subgroup declined more significantly ( P <0.05 or P <0.01).(2) There was positive correlation between NF-κB and IL-6, and between NF-κB and hs CRP ( r =0.550, r =0.675,P <0.01) .Conclusion The activity of NF-κB in patients with diabetes mellitus and coronary heart di-sease was higher, being treated with high dose of atorvastatin can significantly inhibit the inflammatory response in vivo.