CAJ | 학술논문

目的：探究血管内皮生长因子(VEGF)基因多态性与动脉瘤性蛛网膜下腔出血(aSAH)的相关性。方法采用病例对照的研究方法，实验组为158例aSAH患者，均行颅脑CT检查证实为aSAH，并经脑血管造影证实是否存在颅内动脉瘤；对照组为146名健康成年人。取两组外周血，提取基因组DNA。检测VEGF2578C/A的基因型及等位基因分布频率。比较各位点基因型频率的观察值与预期值并进行卡方检验和Hardy-Weinberg平衡分析，以及VEGF基因型与aSAH的相关性。结果①对照组VEGF的2个多态位点的基因型频率分布符合Hardy-Weinberg平衡(P>0.05)。②VEGF2578C/A基因型及等位基因分布，CC+CA基因型频率，实验组显著高于对照组(P<0.05)；实验组的C等位基因频率明显高于对照组，两组比较差异有统计学意义(χ2=4.256，P=0.037)。结论 VEGF基因启动子区-2578C/A多态性可能与aSAH有关。
목적：탐구혈관내피생장인자(VEGF)기인다태성여동맥류성주망막하강출혈(aSAH)적상관성。방법채용병례대조적연구방법，실험조위158례aSAH환자，균행로뇌CT검사증실위aSAH，병경뇌혈관조영증실시부존재로내동맥류；대조조위146명건강성년인。취량조외주혈，제취기인조DNA。검측VEGF2578C/A적기인형급등위기인분포빈솔。비교각위점기인형빈솔적관찰치여예기치병진행잡방검험화Hardy-Weinberg평형분석，이급VEGF기인형여aSAH적상관성。결과①대조조VEGF적2개다태위점적기인형빈솔분포부합Hardy-Weinberg평형(P>0.05)。②VEGF2578C/A기인형급등위기인분포，CC+CA기인형빈솔，실험조현저고우대조조(P<0.05)；실험조적C등위기인빈솔명현고우대조조，량조비교차이유통계학의의(χ2=4.256，P=0.037)。결론 VEGF기인계동자구-2578C/A다태성가능여aSAH유관。
Aim To investigate the correlation between vascular endothelial growth factor (VEGF) gene- polymorphisms and aneurismal subarachnoid hemorrhage (aSAH). Methods Case-control study method was used in this study, the experimental group was composed of 158 cases of aSAH patients and the control group was composed of 146 healthy adults. Patients in experimental group were all conifrmed to have subarachnoid hemorrhage by CT and have aneurysm by DSA. Peripheral blood collected from everyone in both of the groups was used to extract genome DNA in order to detect the genotype and frequency of allele of VEGF. The observed value and expected value of genotype frequency at special locus were compared, and Hardy-Weinberg balance analysis viaχ2 test were done. The correlation of genetype of VEGF and morbidity of aSAH and correlation of genotype of VEGF and subarachnoid hemorrhage were discussed. Results Distribution of VEGF genotype at the two polymorphic sites conformed to H-W balance analysis (P>0.05) in both the control and the experimental group. Frequency of CC and CA in the experimental group was higher than the control group. Conclusion Polymorphism of VEGF-2578C/A may be associated with aneurysmal subarachnoid hemorrhage.