广州医科大学学报
廣州醫科大學學報
엄주의과대학학보
Academic Journal of Guangzhou Medical College
2014年
6期
39-42
,共4页
陈果%陈彦飞%莫汝均%何慧婵%梁宇翔%陈佳鸿%钟惟德
陳果%陳彥飛%莫汝均%何慧嬋%樑宇翔%陳佳鴻%鐘惟德
진과%진언비%막여균%하혜선%량우상%진가홍%종유덕
CCL18%前列腺癌%Gleason评分%实时荧光定量PCR%免疫组化
CCL18%前列腺癌%Gleason評分%實時熒光定量PCR%免疫組化
CCL18%전렬선암%Gleason평분%실시형광정량PCR%면역조화
CCL18%prostate cancer%Gleason score%real ̄time fluorescent quantitative PCR%immunohistochemistry
目的::检测CCL18在良性前列腺增生和前列腺癌中的表达,并分析其表达水平与临床病理特征的关系。方法:通过实时荧光定量PCR检测CCL18基因在前列腺癌、前列腺增生组织中mRNA的表达情况,用免疫组化检测前列腺癌、前列腺增生标本中CCL18蛋白的表达情况。分析CCL18基因的表达水平与临床病理特征的关系。结果:前列腺癌患者组织中CCL18 mRNA显著上调,CCL18蛋白表达与前列腺癌分化程度有正相关的变化趋势, CCL18蛋白表达上调肿瘤发生发展、临床 Gleason 评分相关( P<0.05)。结论:CCL18蛋白表达与前列腺癌分化程度呈正相关趋势,检测CCL18蛋白表达水平可协助判断前列腺癌分化程度并评估预后。
目的::檢測CCL18在良性前列腺增生和前列腺癌中的錶達,併分析其錶達水平與臨床病理特徵的關繫。方法:通過實時熒光定量PCR檢測CCL18基因在前列腺癌、前列腺增生組織中mRNA的錶達情況,用免疫組化檢測前列腺癌、前列腺增生標本中CCL18蛋白的錶達情況。分析CCL18基因的錶達水平與臨床病理特徵的關繫。結果:前列腺癌患者組織中CCL18 mRNA顯著上調,CCL18蛋白錶達與前列腺癌分化程度有正相關的變化趨勢, CCL18蛋白錶達上調腫瘤髮生髮展、臨床 Gleason 評分相關( P<0.05)。結論:CCL18蛋白錶達與前列腺癌分化程度呈正相關趨勢,檢測CCL18蛋白錶達水平可協助判斷前列腺癌分化程度併評估預後。
목적::검측CCL18재량성전렬선증생화전렬선암중적표체,병분석기표체수평여림상병리특정적관계。방법:통과실시형광정량PCR검측CCL18기인재전렬선암、전렬선증생조직중mRNA적표체정황,용면역조화검측전렬선암、전렬선증생표본중CCL18단백적표체정황。분석CCL18기인적표체수평여림상병리특정적관계。결과:전렬선암환자조직중CCL18 mRNA현저상조,CCL18단백표체여전렬선암분화정도유정상관적변화추세, CCL18단백표체상조종류발생발전、림상 Gleason 평분상관( P<0.05)。결론:CCL18단백표체여전렬선암분화정도정정상관추세,검측CCL18단백표체수평가협조판단전렬선암분화정도병평고예후。
Objective:To determine the expression of CCL18 in benign prostate hyperplasia ( BPH) and prostate cancer tissues, and analyze the correlation between the CCL18 expression level and pathological characteristics. Methods:The expression of CCL18 gene in prostate cancer and BPH tissues was determined by real ̄time fluorescent quantitative PCR. The expression of CCL18 protein in prostate cancer and BPH samples was determined by immunohistochemical method. The correlation between the expression level and pathological characteristics of CCL18 gene was analyzed. Results:The expression of CCL18 mRNA was significantly up ̄regluated in the prostate cancer tissues. The expression of CCL18 protein showed a trend of positive correlation with the degree of differentiation in prostate cancer cells. The up ̄regulation of CCL18 was also correlated with occurrence and development of tumor and the clinical Gleason score(P<0.05). Conclusion:There is a trend of positive correlation between the expression CCL18 protein and the differentiation of prostate cancer. Measurement of CCL18 expression can favor the judgment on the differentiation of prostate cancer and the evaluation of prognosis.