CAJ | 학술논문

目的：：探讨葛根素对大鼠心肌缺血再灌注损伤的保护机制。方法：24只雌性Sprague￣Dawley (SD)大鼠随机分为假手术组、缺血再灌注组和葛根素干预组，RT￣PCR和Western blotting法检测心肌中TLR4 mRNA及TLR4蛋白的表达，同时四唑氮蓝染色测定心肌梗死范围。结果：大鼠心肌缺血再灌注损伤模型造模成功；葛根素干预组的心肌梗死范围明显小于缺血再灌注组(P<0.01)，且该组大鼠TLR4 mRNA及蛋白的表达也显著低于缺血再灌注组( P<0.01)。结论：葛根素干预可减轻大鼠心肌缺血再灌注的损伤，其机制可能与抑制TLR4的表达相关。
목적：：탐토갈근소대대서심기결혈재관주손상적보호궤제。방법：24지자성Sprague￣Dawley (SD)대서수궤분위가수술조、결혈재관주조화갈근소간예조，RT￣PCR화Western blotting법검측심기중TLR4 mRNA급TLR4단백적표체，동시사서담람염색측정심기경사범위。결과：대서심기결혈재관주손상모형조모성공；갈근소간예조적심기경사범위명현소우결혈재관주조(P<0.01)，차해조대서TLR4 mRNA급단백적표체야현저저우결혈재관주조( P<0.01)。결론：갈근소간예가감경대서심기결혈재관주적손상，기궤제가능여억제TLR4적표체상관。
Objective:To investigate the mechanism underlying the protective effects of puerarin on myocardial ischemia￣reperfusion injury ( MIRI) in rats. Methods:Twenty￣four female Sprague￣Dawley ( SD) rats were randomly divided into the sham operation group, ischemia￣reperfusion group and puerarin intervention group. The expressions of TLR4 mRNA and protein were assessed by RT￣PCR and Western blotting. Meanwhile, the area of myocardial infarction ( MI) was measured by nitroblue tetrazolium ( NBT) staining. Results: The model of MIRI was successfully built in rats. The MI area in the puerarin intervention group was significantly smaller than that in the ischemia￣reperfusion group ( P<0.01) , and the expressions of TLR4 mRNA and protein were significantly lower than those in the ischemia￣reperfusion group as well (P<0.01). Conclusion: Puerarin intervention can relieve MIRI in rats and its underlying mechanism may be related to the inhibition of TLR4 expression.