柚皮苷对去势大鼠骨质疏松性骨折愈合的作用
유피감대거세대서골질소송성골절유합적작용
Effects of naringin on bone healing of osteoporotic fracture in ovariectomized rats
  • 万方数据
    中华创伤杂志 중화창상잡지
    Chinese Journal of Traumatology
    2015年 4期 370-375 ,共6页

    李风波%孙晓雷%马剑雄%张杨%赵斌%李艳军%韩哲%马信龙

    리풍파%손효뢰%마검웅%장양%조빈%리염군%한철%마신룡

    胫骨骨折%骨质疏松%柚皮苷 脛骨骨摺%骨質疏鬆%柚皮苷
    경골골절%골질소송%유피감
    Tibial fractures%Osteoporosis%Naringin
    目的 观察柚皮苷对去势大鼠骨质疏松骨折愈合的作用,探讨柚皮苷促进大鼠骨质疏松骨折愈合的机制.方法 选择60只3个月龄雌性SD大鼠,行双侧卵巢切除,建立骨质疏松动物模型,2个月后制作右侧胫骨横行骨折.术后按随机数字表法分为对照组(20只)、柚皮苷组(20只)和雌激素组(20只).柚皮苷组灌服柚皮苷100 mg·kg-1·d-1;雌激素组灌服17-β雌二醇22.5μg·kg-1·d-1;对照组给予等体积的等渗盐水皮下注射.术后分别于2,8周处死动物,取出手术侧胫骨,行X线片进行评分;然后行双能X线吸收测定骨折部位骨密度(BMD);采用Micro-CT对骨折部位进行定量分析,检测指标:骨体积(BV)、相对骨体积比(BV/TV)、平均骨小梁厚度(Tb.Th);ELISA法测定血清骨钙素与大鼠Ⅰ型胶原C端肽(CTX-1);三点弯曲力学试验测定骨折最大载荷.结果 术后2周对照组、柚皮苷组、雌激素组X线片评分分别为0.6±0.6,1.2±0.5,1.4±0.6,柚皮苷组、雌激素组均较对照组比较差异有统计学意义(P＜0.05);术后8周对照组、柚皮苷组、雌激素组X线评分分别为2.6±0.6,3.6±0.6,3.6±0.6,柚皮苷组、雌激素组均较对照组比较差异有统计学意义(P＜0.05).术后2周对照组、柚皮苷组、雌激素组BMD值分别为(117.4 ± 4.4) mg/cm2、(129.4±2.8) mg/cm2、(127.6±3.0) mg/cm2,柚皮苷组、雌激素组均较对照组比较差异有统计学意义(P＜0.05);术后8周对照组、柚皮苷组、雌激素组BMD值分别为(119.0±3.8) mg/cm2、(132.0±5.8)mg/cm2、(133.8±3.2) mg/cm2,柚皮苷组、雌激素组均较对照组比较差异有统计学意义(P＜0.05).Micro-CT扫描显示柚皮苷组和雌激素组骨折断端骨小梁数目及骨小梁连续性明显多于对照组.术后2周对照组、柚皮苷组、雌激素组血骨钙素分别为(32.5±2.2)ng/L、(41.5±3.1) ng/L、(21.1 ±2.6)ng/L,任意两组比较差异均有统计学意义(P＜0.05);术后8周对照组、柚皮苷组、雌激素组血骨钙素分别为(16.7±3.8)ng/L、(37.0±2.9)ng/L、(29.7±4.0) ng/L,任意两组比较差异均有统计学意义(P＜0.05).术后2周对照组、柚皮苷组、雌激素组血清CTX-1分别为(4.1±0.6)ng/ml、(3.0±0.8) ng/ml、(3.4±0.4) ng/ml,任意两组比较差异均有统计学意义(P＜0.05);术后8周对照组、柚皮苷组、雌激素组血清CTX-1分别为(3.0±0.7) ng/ml、(2.5±0.5)ng/ml、(2.7±0.3) ng/ml,柚皮苷组、雌激素组均较对照组比较差异有统计学意义(P＜0.05).从2周到8周,所有组骨折的力学强度均随时间的增加而增强,且在2,8周柚皮苷组最用最强,优于对照组与雌激素组(P＜0.05).结论 柚皮苷可提高大鼠骨质疏松骨折BMD、BV、BV/TV、Tb.Th,改善骨代谢,从而提高骨折愈合的力学强度.
    목적 관찰유피감대거세대서골질소송골절유합적작용,탐토유피감촉진대서골질소송골절유합적궤제.방법 선택60지3개월령자성SD대서,행쌍측란소절제,건립골질소송동물모형,2개월후제작우측경골횡행골절.술후안수궤수자표법분위대조조(20지)、유피감조(20지)화자격소조(20지).유피감조관복유피감100 mg·kg-1·d-1;자격소조관복17-β자이순22.5μg·kg-1·d-1;대조조급여등체적적등삼염수피하주사.술후분별우2,8주처사동물,취출수술측경골,행X선편진행평분;연후행쌍능X선흡수측정골절부위골밀도(BMD);채용Micro-CT대골절부위진행정량분석,검측지표:골체적(BV)、상대골체적비(BV/TV)、평균골소량후도(Tb.Th);ELISA법측정혈청골개소여대서Ⅰ형효원C단태(CTX-1);삼점만곡역학시험측정골절최대재하.결과 술후2주대조조、유피감조、자격소조X선편평분분별위0.6±0.6,1.2±0.5,1.4±0.6,유피감조、자격소조균교대조조비교차이유통계학의의(P＜0.05);술후8주대조조、유피감조、자격소조X선평분분별위2.6±0.6,3.6±0.6,3.6±0.6,유피감조、자격소조균교대조조비교차이유통계학의의(P＜0.05).술후2주대조조、유피감조、자격소조BMD치분별위(117.4 ± 4.4) mg/cm2、(129.4±2.8) mg/cm2、(127.6±3.0) mg/cm2,유피감조、자격소조균교대조조비교차이유통계학의의(P＜0.05);술후8주대조조、유피감조、자격소조BMD치분별위(119.0±3.8) mg/cm2、(132.0±5.8)mg/cm2、(133.8±3.2) mg/cm2,유피감조、자격소조균교대조조비교차이유통계학의의(P＜0.05).Micro-CT소묘현시유피감조화자격소조골절단단골소량수목급골소량련속성명현다우대조조.술후2주대조조、유피감조、자격소조혈골개소분별위(32.5±2.2)ng/L、(41.5±3.1) ng/L、(21.1 ±2.6)ng/L,임의량조비교차이균유통계학의의(P＜0.05);술후8주대조조、유피감조、자격소조혈골개소분별위(16.7±3.8)ng/L、(37.0±2.9)ng/L、(29.7±4.0) ng/L,임의량조비교차이균유통계학의의(P＜0.05).술후2주대조조、유피감조、자격소조혈청CTX-1분별위(4.1±0.6)ng/ml、(3.0±0.8) ng/ml、(3.4±0.4) ng/ml,임의량조비교차이균유통계학의의(P＜0.05);술후8주대조조、유피감조、자격소조혈청CTX-1분별위(3.0±0.7) ng/ml、(2.5±0.5)ng/ml、(2.7±0.3) ng/ml,유피감조、자격소조균교대조조비교차이유통계학의의(P＜0.05).종2주도8주,소유조골절적역학강도균수시간적증가이증강,차재2,8주유피감조최용최강,우우대조조여자격소조(P＜0.05).결론 유피감가제고대서골질소송골절BMD、BV、BV/TV、Tb.Th,개선골대사,종이제고골절유합적역학강도.
    Objective To investigate the effect of naringin on bone healing of osteoporotic fracture in ovariectomized rats and the possible mechanism.Methods Sixty female SD rats,3 months of age,were oophorectomized bilaterally before establishment of the osteoporotic model.Two months after the operation,transverse fracture of right tibia was induced.The rats were assigned to control group,naringin group and estrogen group according to the random number table,with 20 rats per group.Rats in naringin and estrogen groups were treated with naringin 100 mg/kg per day and 17-β-estradiol 22.5 μg/kg per day respectively.Instead,isotonic saline solution of the same volume was administered subcutaneously in control group.Animals in each group were killed at postoperative 2 and 8 weeks.The tibia was dissected to perform X-ray review,dual energy X-ray absorptiometry for measuring bone mineral density (BMD),and Micro-CT tomography for quantitative analysis of bone volume (BV),bone volume over total volume (BV/TV) and trabecula thickness (Tb.Th).Serum levels of osteocalcin and C-telopeptide of type Ⅰ collagen (CTX-1) were detected by the Elisa method.Maximum fracture load was tested by the three-point bend test.Results At postoperative 2 and 8 weeks,X-ray score and BMD were significantly higher in naringingroup [X-ray score:(1.2 ±0.5)points,(3.6±0.6)points; BMD:(129.4±2.8)mg/cm2,(132.0 ± 5.8) mg/cm2] and estrogen group [X-ray score:(1.4 ± 0.6) points,(3.6 ± 0.6) points ; BMD:(127.6 ± 3.0) mg/cm2,(133.8 ± 3.2) mg/cm2] than in control group [X-ray score:(0.6 ± 0.6)points,(2.6±0.6)points; BMD:(117.4±4.4)mg/cm2,(119.0±3.8)mg/cm2] (P＜0.05).Micro-CT imaging indicated more trabecular bone and better trabecular continuity in naringin and estrogen groups than in control group.At postoperative 2 and 8 weeks,serum levels of osteocalcin and CTX-1 differed significandy between only two of the groups (P ＜0.05):control group [osteocalcin:(32.5 ±2.2) ng/L,(16.7 ± 3.8) ng/L; CTX-1:(4.1 ± 0.6) ng/ml,(3.0 ± 0.7) ng/ml],naringin group [osteocalcin:(41.5 ± 3.1) ng/L,(37.0 ± 2.9) ng/L; CTX-1:(3.0 ± 0.8) ng/ml,(2.5 ± 0.5) ng/ml] and estrogen group [osteocalcin:(21.1 ± 2.6) ng/L,(29.7 ± 4.0) ng/L; CTX-1:(3.4 ±0.4) ng/ml,(2.7 ± 0.3) ng/ml].Mechanical strength of all groups enhanced over time,with the maximum strength significantly higher in naringin group than in control and estrogen groups (P ＜ 0.05).Conclusion Naringin promotes the mechanical strength in fracture healing by improving the BMD,BV,BV/TV and Tb.Th and ameliorating bone metabolism.
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