CAJ | 학술논문

目的：：探讨中药合剂对种植体周围骨整合的作用机理；方法：健康雄性6m龄新西兰大白兔24只，随机分为对照组(12只)和实验组(12只)。实验组饲料中加入补肾中药合剂，对照组常规饲料。分别喂养3 d后在其胫骨顶端植入纯钛种植体3颗。于种植后30、60、90 d处死相同例数动物( n＝6)。免疫组织化学技术检测OPG、PGL、COX￣2的表达变化；结果：30 d时实验组OPG、OPGL、COX￣2均中度阳性表达，明显高于对照组(弱阳性)，两组间有统计学意义(P<0.05)；60d时实验组OPG、OPGL表达(强度阳性)明显高于对照组(中强阳性)，两组间有统计学意义( P<0.05)，而两组COX￣2均弱阳性表达，两组间没有统计学意义(P>0.05)；90d两组OPG、OPGL、COX￣2均弱阳性表达，两组间没有统计学意义(P>0.05)；结论：OPG、OPGL和COX￣2参与了种植后的骨组织修复过程，而中药合剂能进一步提高种植术后OPG、OPGL和COX￣2的表达水平，提示补肾中药合剂可能促进种植体的周围骨整合。
목적：：탐토중약합제대충식체주위골정합적작용궤리；방법：건강웅성6m령신서란대백토24지，수궤분위대조조(12지)화실험조(12지)。실험조사료중가입보신중약합제，대조조상규사료。분별위양3 d후재기경골정단식입순태충식체3과。우충식후30、60、90 d처사상동례수동물( n＝6)。면역조직화학기술검측OPG、PGL、COX￣2적표체변화；결과：30 d시실험조OPG、OPGL、COX￣2균중도양성표체，명현고우대조조(약양성)，량조간유통계학의의(P<0.05)；60d시실험조OPG、OPGL표체(강도양성)명현고우대조조(중강양성)，량조간유통계학의의( P<0.05)，이량조COX￣2균약양성표체，량조간몰유통계학의의(P>0.05)；90d량조OPG、OPGL、COX￣2균약양성표체，량조간몰유통계학의의(P>0.05)；결론：OPG、OPGL화COX￣2삼여료충식후적골조직수복과정，이중약합제능진일보제고충식술후OPG、OPGL화COX￣2적표체수평，제시보신중약합제가능촉진충식체적주위골정합。
Objective:To investigate the mechanism of traditional Chinese medicine ( TCM ) mixture on osseointegration around implant. Methods: Twenty￣four healthy male New Zealand rabbits ( 6 months of age ) were randomly divided into the control group (n=12) and the experimental group (n=12). The fodder in the experimental group was added with kidney￣tonifying TCM, and the control group was fed with conventional fodder. All animals were implanted with 3 pure titanium implants respectively at the top of the shin bone after 3 days of feeding. Then, the same number of cases( n=6) were executed at 30 d, 60 d and 90 d after implantation. And the change in expression of OPG, OPGL and COX￣2 were determined by immunohistochemical technique. Results:The expression of OPG, OPGL and COX￣2 in the experimental group were moderately positive at 30 d, which were higher than those in the control group ( weakly positive) with statistical significance ( P<0.05) . The expression of OPG and OPGL in the experimental group ( strongly positive) were significantly higher than those in the control group (moderately strong positive) at 60 d with statistical significance (P<0.05). However, the expression of COX￣2 in both groups were positive with no statistical significance ( P>0.05) . All the expression of OPG, OPGL and COX￣2 in both groups were weakly positive at 90 d with no statistical significance (P>0.05). Conclusion:OPG, OPGL and COX￣2 involve in the repair process of bone tissues after implantation, and TCM mixture can further improve the expression levels of OPG, OPGL and COX￣2 after implantation, suggesting kidney￣tonifying TCM may promote the osseointegration around the implant.