CAJ | 학술논문

目的探讨结直肠癌组织中Formin 2(FMN2)蛋白的表达及其与结直肠癌患者临床病理因素和预后的关系.方法回顾性分析2008年1月至2011年12月第四军医大学西京消化病医院收治的335例结直肠癌患者的临床病理资料.收集患者手术切除的结直肠标本进行研究.采用免疫组织化学染色检测结直肠癌患者癌组织及癌旁组织中FMN2蛋白的表达情况,分析其与结直肠癌患者临床病理因素和预后的关系.采用电话或信件方式进行随访,随访时间截至2014年6月.率的比较以及FMN2表达与临床病理因素的关系分析采用x2检验.采用Kaplan-Meier法绘制生存曲线,Log-rank检验进行生存分析.结果 FMN2蛋白的表达位于结直肠癌组织细胞的细胞质.结直肠癌组织中FMN2蛋白的阳性表达率为53.73％ (180/335),癌旁组织中为80.90％(271/335),两者比较,差异有统计学意义(x2=46.792,P＜0.05).结直肠癌组织中FMN2蛋白的表达在结直肠癌患者的肿瘤分化程度、TNM分期及淋巴结转移方面比较,差异有统计学意义(x2=7.707,36.472,40.635,P＜0.05).322例患者获得术后随访,随访率为96.12％(322/335).随访时间为3～36个月,中位随访时间为26个月.其中173例FMN2蛋白阴性表达的结直肠癌患者中位生存时间为30个月,3年累积生存率为48.6％;149例FMN2蛋白阳性表达的结直肠癌患者中位生存时间为36个月,3年累积生存率为68.5％,两者生存情况比较,差异有统计学意义(x2=16.283,P＜0.05).结论 FMN2蛋白在结直肠癌组织中表达下调,与结直肠癌的发生、发展密切相关,可作为判断患者预后的指标.
목적 탐토결직장암조직중Formin 2(FMN2)단백적표체급기여결직장암환자림상병리인소화예후적관계.방법 회고성분석2008년1월지2011년12월제사군의대학서경소화병의원수치적335례결직장암환자적림상병리자료.수집환자수술절제적결직장표본진행연구.채용면역조직화학염색검측결직장암환자암조직급암방조직중FMN2단백적표체정황,분석기여결직장암환자림상병리인소화예후적관계.채용전화혹신건방식진행수방,수방시간절지2014년6월.솔적비교이급FMN2표체여림상병리인소적관계분석채용x2검험.채용Kaplan-Meier법회제생존곡선,Log-rank검험진행생존분석.결과 FMN2단백적표체위우결직장암조직세포적세포질.결직장암조직중FMN2단백적양성표체솔위53.73％ (180/335),암방조직중위80.90％(271/335),량자비교,차이유통계학의의(x2=46.792,P＜0.05).결직장암조직중FMN2단백적표체재결직장암환자적종류분화정도、TNM분기급림파결전이방면비교,차이유통계학의의(x2=7.707,36.472,40.635,P＜0.05).322례환자획득술후수방,수방솔위96.12％(322/335).수방시간위3～36개월,중위수방시간위26개월.기중173례FMN2단백음성표체적결직장암환자중위생존시간위30개월,3년루적생존솔위48.6％;149례FMN2단백양성표체적결직장암환자중위생존시간위36개월,3년루적생존솔위68.5％,량자생존정황비교,차이유통계학의의(x2=16.283,P＜0.05).결론 FMN2단백재결직장암조직중표체하조,여결직장암적발생、발전밀절상관,가작위판단환자예후적지표.
Objective To investigate the expression of Formin 2 protein (FMN2) in the tissues of colorectal cancer (CRC) and relationship between clinicopathologic factors and prognosis of patients.Methods The clinicopathologic data of 335 patients with CRC who were admitted to the Xijing Hospital of Digestive Diseases from January 2008 to December 2011 were retrospectively analyzed.The colorectal specimens from surgical resection were analyzed.The expressions of FMN2 in the cancer and paracarcinoma tissues were examined by the immunohistochemistry.Patients were followed up through telephone interview and correspondence until June 2014.The comparison of rate and relationship between expressions of FMN2 and clinicopathologic factors were analyzed using the chi-square test.The survival curve was generated using the Kaplan-Meier method,and the survival analysis was conducted using the Log-rank test.Results The expression of FMN2 was predominantly detected in the cytoplasm of the tumor cells.The positive rate of FMN2 protein expression in the CRC and paracarcinoma tissues were 53.73％ (180/335) and 80.90％ (271/335),respectively,with a significant difference (x2 =46.792,P ＜0.05).The expression level of FMN2 protein in patients with CRC was however associated with tumor differentiation,TNM stage and lymph node metastasis (x2 =7.707,36.472,40.635,P ＜ 0.05).A total of 322 patients were followed up for 3-36 months (median,26 months) with a follow-up rate of 96.12％ (322/335).The median survival time and 3-year cumulative survival rate in 173 patients with negative expression of FMN2 protein were 30 months and 48.6％,which were compared with 36 months and 68.5％ in 149 patients with positive expression of FMN2 protein,with a statically significance in the survival (x2 =16.283,P ＜ 0.05).Conclusion The low expression of FMN2 protein in the CRC is positively correlated with the occurrence and development of CRC,and it may be a prognostic indicator for patients.