CAJ | 학술논문

为揭示复等位基因遗传的大白菜雄性不育分子机制，通过基于质谱的蛋白质组学同位素相对标记与绝对定量技术技术（ iTRAQ），开展了大白菜核基因雄性不育蛋白质组学研究，以找到不育与可育材料中差异表达蛋白，从蛋白水平来进一步揭示大白菜雄性不育的分子遗传机制。通过研究，共发现了358个差异蛋白，其中可育中上调表达的蛋白有226个，下调表达的蛋白有132个，GO 分析结果表明，鉴定的蛋白质组数据具有较好的生物学功能覆盖范围。通过双向电泳验证，差异蛋白点差异特性与 iTRAQ结果类似，表明 iTRAQ用于差异蛋白分析结果可靠。
위게시복등위기인유전적대백채웅성불육분자궤제，통과기우질보적단백질조학동위소상대표기여절대정량기술기술（ iTRAQ），개전료대백채핵기인웅성불육단백질조학연구，이조도불육여가육재료중차이표체단백，종단백수평래진일보게시대백채웅성불육적분자유전궤제。통과연구，공발현료358개차이단백，기중가육중상조표체적단백유226개，하조표체적단백유132개，GO 분석결과표명，감정적단백질조수거구유교호적생물학공능복개범위。통과쌍향전영험증，차이단백점차이특성여 iTRAQ결과유사，표명 iTRAQ용우차이단백분석결과가고。
To better understand the molecular mechanisms of multiple-allele-inherited male sterility in Chinese cabbage( Brassica rapa L. ssp pekinensis),the iTRAQ quantitation technique based on mass spectrometry were used to study the differencial expression between proteins fertile- and sterile-buds. This research would provide the basis for analyzing the molecular mechanism of multiple-allele-inherited male sterility in Chinese cabbage. In this re-search,a total of 358 differencial expressed proteins(226 up-expressed and 132 down-expressed proteins in fertile buds)were found and Gene Ontology( GO)analysis showed that the proteins identified data has better coverage of biological functions. It is verified by two-dimensional electrophoresis(2-DE). This results is consistent with iTRAQ technique,which indicated that using iTRAQ to analyze protein profile is reliable.