CAJ | 학술논문

通过研究油菜体内过氧化物酶（ POD）、多酚氧化酶（ PPO）、过氧化氢酶（ CAT）、苯丙氨酸解氨酶（ PAL）、超氧化物歧化酶（ SOD）含量及活性变化规律与黑胫病侵染过程、品种抗病性的关系，揭示不同油菜品种对黑胫病菌的抗病机制。用紫外分光光度计测定并比较分析抗、感品种接种前后，各种防御酶活性的变化规律。接种前，抗病品种的 POD、PPO、CAT活性高于感病品种，PAL、SOD活性则与感病品种相近。接种后抗病品种表现出较高的敏感性，5种防御酶活性均迅速升高而达到峰值，且其酶活性增加量显著大于感病品种；而感病品种防御酶的活性或增幅较小或较抗病品种滞后。黑胫病菌可以诱导油菜体内5种防御酶活性变化，POD、PPO、CAT、PAL、SOD 的酶活性与油菜的抗病性具有正相关关系，这些防御酶活性峰值出现的高低与早晚，可作为早期鉴定油菜抗黑胫病的生理指标。
통과연구유채체내과양화물매（ POD）、다분양화매（ PPO）、과양화경매（ CAT）、분병안산해안매（ PAL）、초양화물기화매（ SOD）함량급활성변화규률여흑경병침염과정、품충항병성적관계，게시불동유채품충대흑경병균적항병궤제。용자외분광광도계측정병비교분석항、감품충접충전후，각충방어매활성적변화규률。접충전，항병품충적 POD、PPO、CAT활성고우감병품충，PAL、SOD활성칙여감병품충상근。접충후항병품충표현출교고적민감성，5충방어매활성균신속승고이체도봉치，차기매활성증가량현저대우감병품충；이감병품충방어매적활성혹증폭교소혹교항병품충체후。흑경병균가이유도유채체내5충방어매활성변화，POD、PPO、CAT、PAL、SOD 적매활성여유채적항병성구유정상관관계，저사방어매활성봉치출현적고저여조만，가작위조기감정유채항흑경병적생리지표。
In order to explore the resistance mechanism of different rapeseed varieties to Leptosphaeria biglobo-sa,we analyzed the relation between the changes in contents and activities of defense enzymes peroxidase( POD), polyphemol oxidase( PPO ),catalase ( CAT ),phenylalanine ammonia lyase ( PAL ) and superoxide dismutase ( SOD)in different rapeseed cultivars with the infection process and varieties resistance. The activities of POD, PPO,CAT,PAL and SOD in resistant and susceptible rapeseed varieties before or after infected were measured u-sing spectrophotometer. For uninfected plant,the resistance varieties had a higher activities of POD,PPO,CAT than susceptible varieties and had an almost the same values of PAL,SOD. After inoculation,resistant varieties showed higher sensitivity to Leptosphaeria biglobosa,the activities of five defense enzyme increased rapidly and reached the peak,the increment were significantly greater than susceptible materials. The enzyme activities of susceptible materi-als rise more slowly or later than resistant varieties. Leptosphaeria biglobosa can induced the changes of five defense enzymes in rapeseed,the enzyme activities of POD,PPO,CAT,PAL and SOD had a positive correlation with rape-seed resistance. These results suggested that early rapid increase of enzyme activities might be used as one of physi-ological indices for selection of rapeseed resistant varieties to L. biglobosa.