贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2015年
4期
82-84
,共3页
崔萍%安慧%李美娜
崔萍%安慧%李美娜
최평%안혜%리미나
桑赤锈病%病原菌%微生物%生物防治%拮抗作用
桑赤鏽病%病原菌%微生物%生物防治%拮抗作用
상적수병%병원균%미생물%생물방치%길항작용
mulberry red rust%pathogenic bacteria%microorganism%biological control%antagonism
为寻求生防微生物用于防治桑赤锈病，采用平板对峙法初步筛选农田根际及非根际土壤、贫瘠山地土壤及健康桑叶分离的微生物，用琼脂平板扩散法测定初筛拮抗菌株发酵液的抑菌活性。结果表明：初筛共得到25株菌株对桑赤锈病病原菌有明显的抑菌带，复筛共得到 5株菌株对其具有较强的拮抗作用，以细菌 C53的抑菌效果最好，抑菌率达65．4％。
為尋求生防微生物用于防治桑赤鏽病，採用平闆對峙法初步篩選農田根際及非根際土壤、貧瘠山地土壤及健康桑葉分離的微生物，用瓊脂平闆擴散法測定初篩拮抗菌株髮酵液的抑菌活性。結果錶明：初篩共得到25株菌株對桑赤鏽病病原菌有明顯的抑菌帶，複篩共得到 5株菌株對其具有較彊的拮抗作用，以細菌 C53的抑菌效果最好，抑菌率達65．4％。
위심구생방미생물용우방치상적수병，채용평판대치법초보사선농전근제급비근제토양、빈척산지토양급건강상협분리적미생물，용경지평판확산법측정초사길항균주발효액적억균활성。결과표명：초사공득도25주균주대상적수병병원균유명현적억균대，복사공득도 5주균주대기구유교강적길항작용，이세균 C53적억균효과최호，억균솔체65．4％。
Microorganisms isolated from farmland rhizosphere and non-rhizosphere soil,rhizosphere soil of plants grown in infertile mountainous area and healthy mulberry leaves were preliminarily screened by the agar plate method,and then the bacteriostatic activity of fermentation broth of screened antagonistic strains was determined by the agar plate diffusion method to probe biological control of mulberry red rust. The results showed that 25 preliminarily screened strains have obvious inhibition zones against mulberry red rust and five secondarily screened strains have the stronger antagonism against mulberry red rust.The antibacterial rate of C53 strain can be up to 65.4%.
