华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2015年
2期
72-77
,共6页
祝俊鹏%杨彬%兰喜%柳纪省%马小军
祝俊鵬%楊彬%蘭喜%柳紀省%馬小軍
축준붕%양빈%란희%류기성%마소군
VP1 基因%克隆%DNA测序%序列分析
VP1 基因%剋隆%DNA測序%序列分析
VP1 기인%극륭%DNA측서%서렬분석
VP1 gene%Cloning%DNA sequencing%Sequence analysis
为了深入研究嵴病毒（swKoV）主要结构蛋白基因 VP1，根据 GenBank中已发表的猪嵴病基因序列设计特异性引物，采用 RT-PCR方法扩增猪嵴病毒 CH441株 VP1基因，并对其进行克隆与测序分析。结果表明，swKoV CH441株的 VP1基因为762 bp，与 GenBank已发表的嵴病毒属的15株嵴病毒序列的 VP1基因相比较，swKoV CH441株的VP1基因与其他各毒株 VP1基因的核苷酸同源性为81.5%~90.2%，氨基酸同源性为86.6%~96.9%，进化分析显示，swKoV CH441株与 GS-1株之间的亲缘关系较近。生物信息学分析显示，VP1蛋白理论等电点（ pI）为4.40，理论分子质量为26.9782 kDa；其序列上共发现18个磷酸化位点，分别为Ser（7）、Thr（6）和Tyr（5），而蛋白的磷酸化与信号转导有关，预测该蛋白为一重要的信号转导分子；无信号肽和跨膜区。为进一步开展 swKoV CH441株 VP1基因在遗传变异等方面的研究奠定了理论基础。
為瞭深入研究嵴病毒（swKoV）主要結構蛋白基因 VP1，根據 GenBank中已髮錶的豬嵴病基因序列設計特異性引物，採用 RT-PCR方法擴增豬嵴病毒 CH441株 VP1基因，併對其進行剋隆與測序分析。結果錶明，swKoV CH441株的 VP1基因為762 bp，與 GenBank已髮錶的嵴病毒屬的15株嵴病毒序列的 VP1基因相比較，swKoV CH441株的VP1基因與其他各毒株 VP1基因的覈苷痠同源性為81.5%~90.2%，氨基痠同源性為86.6%~96.9%，進化分析顯示，swKoV CH441株與 GS-1株之間的親緣關繫較近。生物信息學分析顯示，VP1蛋白理論等電點（ pI）為4.40，理論分子質量為26.9782 kDa；其序列上共髮現18箇燐痠化位點，分彆為Ser（7）、Thr（6）和Tyr（5），而蛋白的燐痠化與信號轉導有關，預測該蛋白為一重要的信號轉導分子；無信號肽和跨膜區。為進一步開展 swKoV CH441株 VP1基因在遺傳變異等方麵的研究奠定瞭理論基礎。
위료심입연구척병독（swKoV）주요결구단백기인 VP1，근거 GenBank중이발표적저척병기인서렬설계특이성인물，채용 RT-PCR방법확증저척병독 CH441주 VP1기인，병대기진행극륭여측서분석。결과표명，swKoV CH441주적 VP1기인위762 bp，여 GenBank이발표적척병독속적15주척병독서렬적 VP1기인상비교，swKoV CH441주적VP1기인여기타각독주 VP1기인적핵감산동원성위81.5%~90.2%，안기산동원성위86.6%~96.9%，진화분석현시，swKoV CH441주여 GS-1주지간적친연관계교근。생물신식학분석현시，VP1단백이론등전점（ pI）위4.40，이론분자질량위26.9782 kDa；기서렬상공발현18개린산화위점，분별위Ser（7）、Thr（6）화Tyr（5），이단백적린산화여신호전도유관，예측해단백위일중요적신호전도분자；무신호태화과막구。위진일보개전 swKoV CH441주 VP1기인재유전변이등방면적연구전정료이론기출。
The aim of the study to investigate the main structural protein of the Kobuvirus VP1 gene. According to the sequences of PKV deposited in GenBank,a pair of special primers was designed for amplifying the VP1 gene of swKoV CH441 strain by RT-PCR. The results of sequence analysis showed that the whole VP1 gene of swKoV CH441 strain consisted of 762 bp. Compared with 15 PKV strains which were deposited in GenBank,the homology of nucleotide sequences was 81. 5%~90. 2%,and the homology of deduced amino acids was 86. 6%~96. 9%. Evo-lution analysis indicated that the swKoV CH441 strain was closely related to GS-1 strains. The bioinformatics analy-sis demonstrated that the isoelectric point and molecular weight of non-structural protein VP1 were 4. 40 and 26. 978 2 kDa. The protein had no signal peptide and transmembrane domain. There were 18 phosphorylation sites including 7 Sers,6 Thrs and 5 Tyrs. Protein phosphorylation was concerned with signal transduction,so this protein may be a signaling molecule. The results provided a theoretical foundation for further research on the study of VP1 gene( protein)in the genetic variation.
