CAJ | 학술논문

目的：采用不同方法对维药破布木果基因组DNA进行提取,为破布木果基因组DNA的研究提供参考。方法采用改良CTAB(十六烷基三乙基溴化铵)法和改良SDS(十二烷基磺酸钠)法提取破布木果基因组DNA,并采用分光光度法和琼脂糖凝胶电泳技术检测提取DNA的纯度和浓度。结果分光光度法检测结果表明,用改良CTAB法提取的基因组DNA纯度优于改良SDS法,无蛋白质和RNA污染。琼脂糖凝胶电泳结果表明,2种方法提取得到的基因组DNA电泳图谱均有明显的主带出现,但改良SDS法提取的基因组DNA降解较多。结论改良CTAB法可以提取相对较高质量的破布木果基因组DNA。
목적：채용불동방법대유약파포목과기인조DNA진행제취,위파포목과기인조DNA적연구제공삼고。방법채용개량CTAB(십륙완기삼을기추화안)법화개량SDS(십이완기광산납)법제취파포목과기인조DNA,병채용분광광도법화경지당응효전영기술검측제취DNA적순도화농도。결과분광광도법검측결과표명,용개량CTAB법제취적기인조DNA순도우우개량SDS법,무단백질화RNA오염。경지당응효전영결과표명,2충방법제취득도적기인조DNA전영도보균유명현적주대출현,단개량SDS법제취적기인조DNA강해교다。결론개량CTAB법가이제취상대교고질량적파포목과기인조DNA。
Objective To extract high quality genomic DNA of Cordia dicholoma seeds by using different methods;To provide references for researches on genomic DNA of Cordia dicholoma seeds. Methods Genomic DNA of Cordia dicholoma seeds was extracted through improved CTAB method and improved SDS method. Purity and concentration of obtained DNA were detected by spectrophotometry and agarose gel electrophoresis. Results The results of spectrophotometry showed that the purity of genomic DNA obtained through improved CTAB method was better than improved SDS method. Genomic DNA extracted through improved CTAB method was without protein and RNA pollution. The results of agarose gel electrophoresis showed that electrophoresis of genomic DNA obtained through the two methods both had the main belt. However, genomic DNA extracted through improved SDS method degraded more than improved CTAB method. Conclusion Improved CTAB method can obtain relatively high quality genomic DNA of Cordia dicholoma seeds.