CAJ | 학술논문

目的：探讨百事乐胶囊对慢性应激抑郁模型大鼠海马神经再生相关cAMP-CREB-BDNF信号通路的影响。方法将SD大鼠随机分为空白组、模型组、氟西汀组及百事乐高、中、低剂量组,除空白组外,采用慢性温和不可预见性应激加孤养的方式建立大鼠抑郁模型,各组给予相应药物灌胃,连续21 d。开野实验、糖水消耗实验、定位航行实验及空间搜索实验检测大鼠行为学的变化,ELISA检测大鼠血浆中皮质酮含量,免疫组化检测海马DG、CA3区蛋白激酶A(PKA)、脑源性神经营养因子(BDNF)、环磷腺苷效应元件结合蛋白(CREB)的表达。结果与模型组比较,百事乐高、中剂量组大鼠水平或垂直活动次数及蔗糖偏食度升高(P＜0.05,P＜0.01),百事乐高剂量组大鼠逃避潜伏期、目标象限潜伏时间缩短(P＜0.05),百事乐高、中、低剂量组血浆皮质酮浓度降低(P＜0.05),百事乐高剂量组海马DG、CA3区PKA、CREB、BDNF表达升高(P＜0.05)。结论百事乐胶囊可能通过cAMP-CREB-BDNF信号通路促进海马神经元再生而实现抗抑郁的作用。
목적：탐토백사악효낭대만성응격억욱모형대서해마신경재생상관cAMP-CREB-BDNF신호통로적영향。방법장SD대서수궤분위공백조、모형조、불서정조급백사악고、중、저제량조,제공백조외,채용만성온화불가예견성응격가고양적방식건립대서억욱모형,각조급여상응약물관위,련속21 d。개야실험、당수소모실험、정위항행실험급공간수색실험검측대서행위학적변화,ELISA검측대서혈장중피질동함량,면역조화검측해마DG、CA3구단백격매A(PKA)、뇌원성신경영양인자(BDNF)、배린선감효응원건결합단백(CREB)적표체。결과여모형조비교,백사악고、중제량조대서수평혹수직활동차수급자당편식도승고(P＜0.05,P＜0.01),백사악고제량조대서도피잠복기、목표상한잠복시간축단(P＜0.05),백사악고、중、저제량조혈장피질동농도강저(P＜0.05),백사악고제량조해마DG、CA3구PKA、CREB、BDNF표체승고(P＜0.05)。결론백사악효낭가능통과cAMP-CREB-BDNF신호통로촉진해마신경원재생이실현항억욱적작용。
Objective To investigate effects of Baishile Capsules on cAMP-CREB-BDNF signal pathway about hippocampal neurogenesis in model rats with chronic unpredicted stress depression. Methods SD rats were randomly divided into blank group, model group, fluoxetine hydrochloride group, and Baishile Capsules high, medium, and low dose groups. Chronic stress depression rat model was established by chronic and mild unpredictable stressors. All groups were given relevant medicine for 21 days. The open-field test, sugar consumption experiment, place navigation test, and space searching experiment were used to detect behavior changes of the rats. ELISA was used to detect content of corticosterone in plasma. The protein expressions of PKA, BDNF, and CREB were detected by immunohistochemical method. Results Compared with model group, Baishile Capsules high dose and medium dose groups could remarkably increase the number of vertical and horizontal activities and 1% sucrose partial eclipse. Platform latency and target quadrant searching time decreased significantly in Baishile Capsules high dose group (P<0.05), and content of corticosterone in plasma increased obviously (P<0.05) in Baishile Capsules all dose groups. Protein expressions of PKA, CREB, and BDNF in hippocampal DG and CA3 area increased significantly (P<0.05) in Baishile Capsules high dose group. Conclusion Baishile Capsules can promote hippocampal neurogenesis through the cAMP-CREB-BDNF signal pathway and realize anti-depression effect.