CAJ | 학술논문

目的：从脑-肠轴角度探讨针刺不同穴位对大鼠应激性胃黏膜损伤的愈合及睡眠情况的影响。方法将雄性Wistar大鼠随机分为空白组、模型组、治胃组、安神组、联合组,每组8只。采用无水乙醇灌胃方法造成应激性胃黏膜损伤大鼠模型,治胃组穴选双侧“足三里”“中脘”,安神组穴选双侧“申脉”“照海”,联合组穴选双侧“足三里”“中脘”“申脉”“照海”,用0.22 mm×13 mm不锈钢毫针刺入5～10 mm,留针20 min。针刺干预5 d后检测热痛阈的变化。按40 mg/kg大鼠体质量腹腔注射2%戊巴比妥钠,记录注射后大鼠的睡眠持续时间。肉眼观察大鼠的胃黏膜形态,Guth法计算黏膜损伤指数(UI),高效液相色谱-电化学检测法测纹状体中5-羟色胺(5-HT)、多巴胺(DA)的含量。结果与空白组比较,模型组热痛阈和睡眠持续时间显著下降(P＜0.01),UI 及5-HT、DA 含量显著升高(P＜0.05,P＜0.01)；与模型组比较,各治疗组热痛阈和睡眠持续时间增加(P＜0.05,P＜0.01),UI均显著降低(P＜0.01),治胃组和联合组中5-HT含量明显降低、治胃组DA含量显著降低(P＜0.01)；各治疗组间比较,联合组热痛阈及睡眠持续时间显著增加(P＜0.01),UI显著降低(P＜0.05)。结论针刺足三里、中脘、照海、申脉可促进大鼠胃黏膜损伤的愈合,同时延长睡眠持续时间,其中四穴联合应用效果更佳,其机制可能是通过调节纹状体中5-HT及DA含量发挥作用。
목적：종뇌-장축각도탐토침자불동혈위대대서응격성위점막손상적유합급수면정황적영향。방법장웅성Wistar대서수궤분위공백조、모형조、치위조、안신조、연합조,매조8지。채용무수을순관위방법조성응격성위점막손상대서모형,치위조혈선쌍측“족삼리”“중완”,안신조혈선쌍측“신맥”“조해”,연합조혈선쌍측“족삼리”“중완”“신맥”“조해”,용0.22 mm×13 mm불수강호침자입5～10 mm,류침20 min。침자간예5 d후검측열통역적변화。안40 mg/kg대서체질량복강주사2%무파비타납,기록주사후대서적수면지속시간。육안관찰대서적위점막형태,Guth법계산점막손상지수(UI),고효액상색보-전화학검측법측문상체중5-간색알(5-HT)、다파알(DA)적함량。결과여공백조비교,모형조열통역화수면지속시간현저하강(P＜0.01),UI 급5-HT、DA 함량현저승고(P＜0.05,P＜0.01)；여모형조비교,각치료조열통역화수면지속시간증가(P＜0.05,P＜0.01),UI균현저강저(P＜0.01),치위조화연합조중5-HT함량명현강저、치위조DA함량현저강저(P＜0.01)；각치료조간비교,연합조열통역급수면지속시간현저증가(P＜0.01),UI현저강저(P＜0.05)。결론침자족삼리、중완、조해、신맥가촉진대서위점막손상적유합,동시연장수면지속시간,기중사혈연합응용효과경가,기궤제가능시통과조절문상체중5-HT급DA함량발휘작용。
Objective To observe the effects of acupuncture on the sleeping quality of male Wistar rats with stress induced gastric mucosal injury from the viewpoint of the brain-intestine axis. Methods Male Wistar rats were randomized into normal control, model, ST36-CV12, BL62-KI6, and combined groups, with 8 rats in each group. The model was established by using intragastric perfusion of ethanol in the rats. Acupoints of Zusanli (ST36) and Zhongwan (CV12) on both sides were used for the ST36-CV12 group. Acupoints of Shenmai (BL62) and Zhaohai (KI6) on both sides were used for the BL62-KI6 group. All four acupoints were used for the combined group. 0.22 mm × 13 mm stainless steel filiform needles were inserted 5-10 mm deep, and were left untouched for 20 minutes before withdrawal. The acupoints were stimulated each day for five days and the paw withdraw thermal latency (PWTL) was detected after the last stimulation. 2%pentobarbital sodium was then injected (40 mg/kg), and sleeping time of rats was detected. Gastric mucosa was observed through naked eyes. Gastric mucosal ulcer Index was determined by using the Guth method. 5-HT and DA contents were detected by using HPLC-ECD method. Results Compared with the normal control group, PWTL and sleeping time decreased (P<0.01), and UI, 5-HT, and DA content increased in model group (P<0.05, P<0.01). Compared with the model group, PTWL and sleeping time increased (P<0.05, P<0.01), and UI decreased in all treatment groups (P<0.01). 5-HT content in ST36-CV12 and combined groups decreased significantly, and DA content in ST36-CV12 group dropped (P<0.01). Compared with other treatment groups, PWTL and sleeping time increased significantly (P<0.01), and UI decreased obviously in combined group (P<0.01). Conclusion Stimulating acupoints of Zusanli, Zhongwan, Shenmai, and Zhaohai (KI6) may promote the recovery of gastric mucosal injury while at the same time increase sleeping time, with four acupoints used in combination having the best efficacy. The mechanism may be related to adjusting contents of 5-HT and DA in corpus striatum.