CAJ | 학술논문

为了探明板栗及其近缘种的亲缘关系并分析栗属的遗传多样性，基于叶绿体微卫星标记技术，选用4对呈现多态性的cpSSR引物，对板栗及其近缘种、野生种共6个种，56份材料进行遗传结构、遗传关系等分析。4个位点扩增等位基因数（Na）平均为3.25，有效等位基因数（Ne）平均为2.554，期望杂合度（He）平均为0.606，群体 Nei’s遗传多样性（Hs）为0.320，各遗传参数值均低于核基因组对群体研究的相应值。另外，各种之间有丰富的 cpSSR 多样性，尤以野生板栗多样性指数最高。结果表明，我国天然野生板栗群体内蕴含更丰富的遗传变异，为我国板栗野生种质保育及可持续开发利用提供了基础数据和科学依据。
위료탐명판률급기근연충적친연관계병분석률속적유전다양성，기우협록체미위성표기기술，선용4대정현다태성적cpSSR인물，대판률급기근연충、야생충공6개충，56빈재료진행유전결구、유전관계등분석。4개위점확증등위기인수（Na）평균위3.25，유효등위기인수（Ne）평균위2.554，기망잡합도（He）평균위0.606，군체 Nei’s유전다양성（Hs）위0.320，각유전삼수치균저우핵기인조대군체연구적상응치。령외，각충지간유봉부적 cpSSR 다양성，우이야생판률다양성지수최고。결과표명，아국천연야생판률군체내온함경봉부적유전변이，위아국판률야생충질보육급가지속개발이용제공료기출수거화과학의거。
The objective of the study is to elucidate the genetic relationship and genetic diversity of Castanea species. Based on the technology of chloroplast microsatellite markers,of total 24 cpSSR primer pairs screened,only four presented length polymorphism in the Castanea nollissina tested. We surveyed 6 species,altogether 56 materi-als with cpSSR markers in order to estimate genetic variation and genetic structure. In the castanea plants,average number of alleles( Na),effective number of alleles( Ne),expected heterozygosity( He)and Nei′s were 3. 25, 2. 554,0. 606 and 0. 320,respectively. Apparently,the parameters of cpSSR loci were much lower than those re-vealed by nuclear SSRs. Furthermore,the intra-specific genetic diversity was high but varied greatly among different species,the wild chestnut in China showed the highest genetic diversity,which one was rich in heredity and varia-tion. The results have important implications of understanding the population genetics and evolutionary patterns in the genus Castanea and provide baseline data for formulating the conservation and management strategies.