华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2015年
2期
140-144
,共5页
张立杰%麻艳超%李康%东方阳%王建设
張立傑%痳豔超%李康%東方暘%王建設
장립걸%마염초%리강%동방양%왕건설
黄瓜%营养器官%果实%苦味基因%SSR标记鉴定
黃瓜%營養器官%果實%苦味基因%SSR標記鑒定
황과%영양기관%과실%고미기인%SSR표기감정
Cucumber%Foliage%Fruit%Bitter gene%SSR markers identification
鉴定无苦味种质,选育无苦味品种是控制黄瓜苦味、改善品质的有效方法。利用黄瓜营养器官苦味基因的标记 SSR02309和果实苦味基因的标记 SSR10795,对78份种质资源进行苦味基因型分析,表明黄瓜种质间和种质内苦味基因的标记基因型存在遗传变异性。明确了78份种质苦味基因的标记基因型。初步鉴定出7份无苦味种质。在田间逆境胁迫条件下,利用感官品尝方法对标记鉴定的7份无苦味种质的苦味性状进行了感官品尝鉴定,结果与标记鉴定的一致。为无苦味黄瓜品种改良奠定了技术与种质基础。
鑒定無苦味種質,選育無苦味品種是控製黃瓜苦味、改善品質的有效方法。利用黃瓜營養器官苦味基因的標記 SSR02309和果實苦味基因的標記 SSR10795,對78份種質資源進行苦味基因型分析,錶明黃瓜種質間和種質內苦味基因的標記基因型存在遺傳變異性。明確瞭78份種質苦味基因的標記基因型。初步鑒定齣7份無苦味種質。在田間逆境脅迫條件下,利用感官品嘗方法對標記鑒定的7份無苦味種質的苦味性狀進行瞭感官品嘗鑒定,結果與標記鑒定的一緻。為無苦味黃瓜品種改良奠定瞭技術與種質基礎。
감정무고미충질,선육무고미품충시공제황과고미、개선품질적유효방법。이용황과영양기관고미기인적표기 SSR02309화과실고미기인적표기 SSR10795,대78빈충질자원진행고미기인형분석,표명황과충질간화충질내고미기인적표기기인형존재유전변이성。명학료78빈충질고미기인적표기기인형。초보감정출7빈무고미충질。재전간역경협박조건하,이용감관품상방법대표기감정적7빈무고미충질적고미성상진행료감관품상감정,결과여표기감정적일치。위무고미황과품충개량전정료기술여충질기출。
Identifying non-bitter germplasms and breeding non-bitter varieties are effective to control bitterness as well as to improve qualities in cucumber. In this study,SSR02309 marker linked to foliage bitter gene and SSR10795 marker linked to fruit bitter gene were used to identify the marker genotypes of 78 cucumber germplasm resources. The result showed genetic variation of marker genotypes exist among different germplasms and within same germplasms as well. The marker genotypes of 78 germplasms were confirmed,and 7 non-bitter cucumber varie-ties were preliminary identified by using the 2 markers. The sensory evaluation results by tasting such 7 cucumber varieties were consistent with the molecular identification results. This study laid technical and germplasm founda-tion for improving non-bitter cucumber varieties.