贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2015年
4期
146-149
,共4页
李平%燕志宏%张芸%陈伟
李平%燕誌宏%張蕓%陳偉
리평%연지굉%장예%진위
H-FABP 基因%宗地花猪%实时荧光定量 PCR
H-FABP 基因%宗地花豬%實時熒光定量 PCR
H-FABP 기인%종지화저%실시형광정량 PCR
heart fatty acid binding protein gene (H-FABP gene)%Zongdi spotted pig%real-time fluo-rescence quantification PCR
为有效提高宗地花猪的肉品质，合理评价养殖模式提供理论依据，参照 GenBank 中猪H-FABP 基因序列设计引物，以猪18S RNA 为内参基因，应用实时荧光定量 PCR 方法检测 H-FABP 基因在不同饲养条件下宗地花猪不同组织器官中的表达量。结果表明：H-FABP 基因在肺脏、心脏、肾脏、小肠、脾脏、肝脏、背最长肌、腰大肌中均有表达，但不同饲养条件下其表达水平各异，放养型背最长肌＞腰大肌＞肾脏＞心脏＞小肠＞肺脏＞脾脏＞肝脏，背最长肌中表达量显著高于其他组织（P ＜0．05）；圈养型腰大肌＞脾脏＞心脏＞肺脏＞小肠＞肝脏＞背最长肌＞肾脏，腰大肌中表达量显著高于其他组织（P ＜0．05），肝脏中表达量高于背最长肌（P ＞0．05）。两种饲养条件下宗地花猪肺脏、肝脏中表达量相当，而在心脏、肾脏、小肠、脾脏、背最长肌中表达差异较大。
為有效提高宗地花豬的肉品質，閤理評價養殖模式提供理論依據，參照 GenBank 中豬H-FABP 基因序列設計引物，以豬18S RNA 為內參基因，應用實時熒光定量 PCR 方法檢測 H-FABP 基因在不同飼養條件下宗地花豬不同組織器官中的錶達量。結果錶明：H-FABP 基因在肺髒、心髒、腎髒、小腸、脾髒、肝髒、揹最長肌、腰大肌中均有錶達，但不同飼養條件下其錶達水平各異，放養型揹最長肌＞腰大肌＞腎髒＞心髒＞小腸＞肺髒＞脾髒＞肝髒，揹最長肌中錶達量顯著高于其他組織（P ＜0．05）；圈養型腰大肌＞脾髒＞心髒＞肺髒＞小腸＞肝髒＞揹最長肌＞腎髒，腰大肌中錶達量顯著高于其他組織（P ＜0．05），肝髒中錶達量高于揹最長肌（P ＞0．05）。兩種飼養條件下宗地花豬肺髒、肝髒中錶達量相噹，而在心髒、腎髒、小腸、脾髒、揹最長肌中錶達差異較大。
위유효제고종지화저적육품질，합리평개양식모식제공이론의거，삼조 GenBank 중저H-FABP 기인서렬설계인물，이저18S RNA 위내삼기인，응용실시형광정량 PCR 방법검측 H-FABP 기인재불동사양조건하종지화저불동조직기관중적표체량。결과표명：H-FABP 기인재폐장、심장、신장、소장、비장、간장、배최장기、요대기중균유표체，단불동사양조건하기표체수평각이，방양형배최장기＞요대기＞신장＞심장＞소장＞폐장＞비장＞간장，배최장기중표체량현저고우기타조직（P ＜0．05）；권양형요대기＞비장＞심장＞폐장＞소장＞간장＞배최장기＞신장，요대기중표체량현저고우기타조직（P ＜0．05），간장중표체량고우배최장기（P ＞0．05）。량충사양조건하종지화저폐장、간장중표체량상당，이재심장、신장、소장、비장、배최장기중표체차이교대。
The expression quantity of H-FABP gene in different organs of Zongdi spotted pig under different feeding patterns was detected by real-time fluorescence quantification PCR method with specific designed primers according to swine H-FABP gene in GenBank and by using swine 18S RNA as the reference gene to provide the theoretical basis for effectively improving meat quality of Zongdi spotted pig and rationally evaluating breeding pattern.The results showed that H-FABP gene has expression in lung, heart,kidney,small intestine,spleen,liver,longissimus muscle and psoas,but there is difference in the expression level between two different breeding patterns.The H-FABP expression quantity in different organs of Zongdi spotted pig under the stocking pattern is longissimus muscle>psoas>kidney>heart>small intestine>lung>spleen>liver and the H-FABP expression quantity in longissimus muscle is higher than other organs significantly(P <0.05).The H-FABP expression quantity in different organs of Zongdi spotted pig under the captive pattern is psoas> spleen>heart>lung> small intestine> liver>longissimus muscle>kidney.The H-FABP expression quantity in psoas is higher than other organs significantly(P <0.05)and the H-FABP expression quantity in liver is higher than longissimus muscle (P >0.05).There is no obvious difference in H-FABP expression quantity of lung and liver between two feeding patterns but difference in H-FABP expression quantity of heart,kidney,small intestine,spleen and longissimus muscle between two feeding patterns.
