CAJ | 학술논문

为筛选出最适蜜环菌栽培生产种的培养基，通过生产种培养基组分筛选试验，测量第20天和第40天时菌丝或菌索的生长速度及其胞外酶活性并进行比较。结果表明：在配方1（70％阔叶木屑，0％棉籽壳，17％麦麸，10％玉米面，1％蔗糖，0．3％磷酸二氢钾，0．2％硫酸镁，0．5％石灰，1％石膏，m水分∶m配料＝1∶1．3）中生长速度最大且最先长满管，且菌丝洁白，与其余不同培养料配方之间生长速度差异显著，而且栽培种培养基含木屑越多，菌株活力越好，越不易形成老化的红褐色菌索；在不同培养料和不同生长阶段其木聚糖酶活性大于 CMC 酶和漆酶，漆酶活性最小，为十位级，其他2个酶为百位级；不同培养基中蜜环菌菌丝分泌的胞外酶活性具有显著性差异，但同种酶在不同培养料中酶活性的变化趋势一致。说明，配方1 可作为蜜环菌适宜的栽培种培养基；不同培养料对胞外酶活性变化趋势影响小，但对其活性影响较大。
위사선출최괄밀배균재배생산충적배양기，통과생산충배양기조분사선시험，측량제20천화제40천시균사혹균색적생장속도급기포외매활성병진행비교。결과표명：재배방1（70％활협목설，0％면자각，17％맥부，10％옥미면，1％자당，0．3％린산이경갑，0．2％류산미，0．5％석회，1％석고，m수분∶m배료＝1∶1．3）중생장속도최대차최선장만관，차균사길백，여기여불동배양료배방지간생장속도차이현저，이차재배충배양기함목설월다，균주활력월호，월불역형성노화적홍갈색균색；재불동배양료화불동생장계단기목취당매활성대우 CMC 매화칠매，칠매활성최소，위십위급，기타2개매위백위급；불동배양기중밀배균균사분비적포외매활성구유현저성차이，단동충매재불동배양료중매활성적변화추세일치。설명，배방1 가작위밀배균괄의적재배충배양기；불동배양료대포외매활성변화추세영향소，단대기활성영향교대。
In order to select the most suitable culture medium of A.mellea,the production of medium component was screened by measuring the A.hypha or bacteria growth speed and the extracellular enzyme activity,the authors compared between 20 and 40 days of hypha or rhizomorph of growth speed and extracellular enzyme activity.Results:Growth rate of the hyphae was the biggest and was the first full of tube and white in the formula(broadleaf sawdust 70% ,cotton seed shell 0,wheat bran 17%,corn flour 10%,sugar 1%,potassium dihydrogen phosphate 0.3%,magnesium sulfate 0.2%,lime 0.5%,gypsum 1%,mwater∶mmaterial=1∶1.3).The growth rate has obvious difference in different nutrient formula,and in culture medium,the more wood hyphae vitality the better,the more difficult to form a red brown rhizomorph;in different culture medium and different growth stages of the activity of xylanase and laccase enzyme is greater than CMC and laccase,the laccase activity is minimum for ten level,others for hundreds of level.Extracellular enzyme activity of A.mellea mycelia secretion of different medium has obvious difference,but the change trend in different culture medium in the same enzyme activity is consistent. Suggests that this formula can be used as armillaria suitable culture medium;different culture material has little effect on the enzyme activity changes in trend,but great influence on the activity of the extracellular enzyme.