CAJ | 학술논문

构建大白菜分子遗传图谱，为大白菜干烧心 QTL定位和分析奠定基础。以大白菜感病品种 B120和大白菜抗病品种黑227为亲本建立的DH系为作图群体，基于所筛选出的74对InDel标记和37对SSR标记构建分子遗传图谱。利用 JoinMap 4.0软件，初步构建了一张覆盖基因组长度为1004.7 cM、平均图距为9.30 cM 的大白菜遗传连锁图，该图谱包含12个连锁群、108个标记位点。该图谱能有效地用于大白菜干烧心 QTL定位。
구건대백채분자유전도보，위대백채간소심 QTL정위화분석전정기출。이대백채감병품충 B120화대백채항병품충흑227위친본건립적DH계위작도군체，기우소사선출적74대InDel표기화37대SSR표기구건분자유전도보。이용 JoinMap 4.0연건，초보구건료일장복개기인조장도위1004.7 cM、평균도거위9.30 cM 적대백채유전련쇄도，해도보포함12개련쇄군、108개표기위점。해도보능유효지용우대백채간소심 QTL정위。
A molecular genetic map of Chinese cabbage was constructed,whose aim that the location and analy-sis of QTL of tipburn in Chinese. The map was constructed based on 74 InDels markers and 37 SSRs markers using DNA samples extracted from a doubled haploid population obtained through microspore culture from the F1 between two homozygous parents:B120 and Black 227. By using JoinMap 4. 0 software analysis,the map consisted 12 linkage groups,included 108 genetic markers and covered 1 004. 7 cM with an average distance of 9. 30 cM. The map would be used effectively to QTL location of tipburn in Chinese cabbage.