CAJ | 학술논문

为研究不同剂量的苜蓿素对AA肉仔鸡免疫指标的影响，选取1日龄AA肉仔鸡120只，随机分为4组，对照组饲喂基础日粮，试验Ⅰ~Ⅲ组基础日粮中分别添加150、300、450 mg/kg苜蓿素。分别于28、35和42 d从每组取10只鸡进行采血，测定血清球蛋白含量；取法氏囊和脾脏，称重并计算免疫器官指数；采用MTT比色法测定T、B淋巴细胞增殖能力。结果表明，基础日粮中添加300 mg/kg苜蓿素饲喂28 d，能够显著提高AA肉仔鸡法氏囊指数（P<0.05）；添加450 mg/kg苜蓿素饲喂28 d，能够显著提高AA肉仔鸡血清球蛋白含量和免疫器官指数（P<0.05）。添加300 mg/kg苜蓿素饲喂35 d，能够显著提高AA肉仔鸡血清球蛋白含量、免疫器官指数（P<0.05），显著促进B淋巴细胞增殖（P<0.05）；添加450 mg/kg苜蓿素饲喂35 d，能够显著提高血清球蛋白含量、脾脏指数（P<0.05），显著促进T淋巴细胞增殖（P<0.05），极显著促进B淋巴细胞增殖（P<0.01）。添加300和450 mg/kg苜蓿素饲喂42 d，能够极显著提高AA肉仔鸡法氏囊指数（P<0.01）；添加450 mg/kg苜蓿素饲喂42 d，能够显著促进B淋巴细胞增殖（P<0.05）。结果提示，日粮中添加苜蓿素能够提高多项AA肉仔鸡免疫指标，以添加450 mg/kg效果最优。
위연구불동제량적목숙소대AA육자계면역지표적영향，선취1일령AA육자계120지，수궤분위4조，대조조사위기출일량，시험Ⅰ~Ⅲ조기출일량중분별첨가150、300、450 mg/kg목숙소。분별우28、35화42 d종매조취10지계진행채혈，측정혈청구단백함량；취법씨낭화비장，칭중병계산면역기관지수；채용MTT비색법측정T、B림파세포증식능력。결과표명，기출일량중첨가300 mg/kg목숙소사위28 d，능구현저제고AA육자계법씨낭지수（P<0.05）；첨가450 mg/kg목숙소사위28 d，능구현저제고AA육자계혈청구단백함량화면역기관지수（P<0.05）。첨가300 mg/kg목숙소사위35 d，능구현저제고AA육자계혈청구단백함량、면역기관지수（P<0.05），현저촉진B림파세포증식（P<0.05）；첨가450 mg/kg목숙소사위35 d，능구현저제고혈청구단백함량、비장지수（P<0.05），현저촉진T림파세포증식（P<0.05），겁현저촉진B림파세포증식（P<0.01）。첨가300화450 mg/kg목숙소사위42 d，능구겁현저제고AA육자계법씨낭지수（P<0.01）；첨가450 mg/kg목숙소사위42 d，능구현저촉진B림파세포증식（P<0.05）。결과제시，일량중첨가목숙소능구제고다항AA육자계면역지표，이첨가450 mg/kg효과최우。
In order to investigate the effect of tricin on immunological indicators of AA broiler, a total of 120 AA broilers were selected and randomly divided into four groups. The control group was fed with basal diet. The basal diet of experimental groupⅠ,ⅡandⅢwere supplemented with 150, 300 and 450 mg/kg tricin, respectively. Ten broilers were chosen from each group in 28th, 35th and 42th d to determine their serum globulin content. Meanwhile, the bursa of Fabricius and spleen of the selected broilers were collected and weighed to calculate their immune organs index, and the proliferation ability of T and B lymphocyte were determined by using MTT colorimetry. The results showed that the index of bursa of Fabricius of AA broilers were significantly increased when they were fed with the basal diet supplemented with 300 mg/kg of tricin for 28 d (P<0.05). The serum globulin level and immune organs index of AA broilers were significantly increased when they were fed with the basal diet supplemented with 450 mg/kg of tricin for 28 d (P<0.05). The serum globulin level, immune organs index and the proliferation ability of B lymphocyte of AA broilers were significantly increased when they were fed with the basal diet supplemented with 300 mg/kg of tricin for 35 d (P<0.05). The serum globulin level, spleen index and the proliferation ability of T and B lymphocyte of AA broilers were significantly increased when they were fed with the basal diet supplemented with 450 mg/kg of tricin for 35 d (P<0.05 or P<0.01). The index of bursa of Fabricius of AA broilers were significantly increased when they were fed with the basal diet supplemented with 300 and 450 mg/kg of tricin for 42 d (P<0.01), respectively. The proliferation ability of B lymphocyte of AA broilers were significantly increased when they were fed with the basal diet supplemented with 450 mg/kg of tricin for 42 d (P<0.05). It was indicated that a series of immunological indicators of AA broiler were increased when they were fed with the basal diet supplemented with tricin, and the optimum adding quantity was 450 mg/kg.