CAJ | 학술논문

通过筛选栽培花生间具有多态性的 SSR，分析总结多态性 SSR 的特征，旨在选择性利用 SSR 标记，推动栽培种花生遗传图谱构建、相关农艺性状的 QTL定位和分子标记辅助育种进程。以秦皇岛光秧、石腰豆、印度窄叶、抗019、四粒红和黑花生6个不同植物学类型的中国栽培种花生为材料，选用不同设计类型的1523个 SSR 标记筛选品种间多态性 SSR，并对其扩增片段长度、重复基序、重复次数等进行分析。结果表明，1523个标记中，筛选获得43对多态性 SSR标记，产生123个多态性标记位点，多态性标记数占2.8%。43对多态性 SSR 标记中，以 ARS ××的多态性最高，在6个品种中多态率达6.8%。统计各种质间具多态性的 SSR，其中石腰豆与四粒红间多态性最高，共筛选到56个多态性标记位点；秦皇岛光秧与印度窄叶间多态率最低，筛选到21个多态性标记位点。在123个多态性 SSR标记位点中，80%多态性 SSR位点片段长度为150~300 bp；18.8%多态标记的重复基序为 GA，其次为 AG、TC，分别占12.5%，12.5%；多态标记中二核苷酸、三核苷酸重复基序的重复次数在15次以上的占60%。
통과사선재배화생간구유다태성적 SSR，분석총결다태성 SSR 적특정，지재선택성이용 SSR 표기，추동재배충화생유전도보구건、상관농예성상적 QTL정위화분자표기보조육충진정。이진황도광앙、석요두、인도착협、항019、사립홍화흑화생6개불동식물학류형적중국재배충화생위재료，선용불동설계류형적1523개 SSR 표기사선품충간다태성 SSR，병대기확증편단장도、중복기서、중복차수등진행분석。결과표명，1523개표기중，사선획득43대다태성 SSR표기，산생123개다태성표기위점，다태성표기수점2.8%。43대다태성 SSR 표기중，이 ARS ××적다태성최고，재6개품충중다태솔체6.8%。통계각충질간구다태성적 SSR，기중석요두여사립홍간다태성최고，공사선도56개다태성표기위점；진황도광앙여인도착협간다태솔최저，사선도21개다태성표기위점。재123개다태성 SSR표기위점중，80%다태성 SSR위점편단장도위150~300 bp；18.8%다태표기적중복기서위 GA，기차위 AG、TC，분별점12.5%，12.5%；다태표기중이핵감산、삼핵감산중복기서적중복차수재15차이상적점60%。
Through screening polymorphic SSRs and analyzing its characteristic,it is anticipated that the identi-fied set of highly informative markers should be very useful to initiate constructing SSR genetic linkage map,QTL research,and molecular assistant breeding studies in cultivated groundnut. A total of 1 523 markers designed by dif-ferent methods were used to screen for polymorphism in 6 peanut accessions with different botanical types,and the characteristics of polymorphic SSR were analyzed in the aspect of amplified fragment length,repeat motif,and repeat number. The result showed that only 43(2. 8%)pairs of markers with 123 polymorphic loci expressed polymor-phism. Among the 43 polymorphic SSRs,ARS × × showed the highest polymorphic rate(6. 8%). For the combina-tion examined,Shiyaodou and Silihong were the highest with polymorphic loci of 56,while Qinhuangdaoguangyang and Yinduzhaiye were the lowest with polymorphic loci of 21. Fragment length of 80% polymorphic loci were be-tween 150 bp and 300 bp;GA motif(18. 8%)was the most abundant,followed by AG(12. 5%)and TC(12. 5%);the repeat number of 60% of di-nucleotide and tri-nucleotide motif was over 15.