CAJ | 학술논문

为探究基因 Pi1与 Pi9的稻瘟病抗性，及基因聚合对感病受体材料的抗性改良情况，以 P121、P32作为供体亲本，保持系金23B 作为受体亲本，通过常规杂交，回交手段，结合分子标记技术（MAS），导入Pi1、Pi9的双基因聚合材料，然后对此聚合材料和分别携带稻瘟病抗性基因 Pi1、Pi9的籼型水稻材料 P121、P32，保持系金23B 进行稻瘟病温室人工接种鉴定及大田自然诱发鉴定。结果表明：受体亲本金23B 叶瘟6级感病，穗颈瘟9级高感，单基因供体亲本 P121、P32叶瘟病级均为4级中感，穗颈瘟病级分别为3级中抗，7级感病，而以金23B 为背景的抗性基因聚合材料，叶瘟病级2级，达抗级水平，穗颈瘟病级3级，达中抗水平，相较于受体亲本和单基因供体亲本，双基因聚合材料对叶瘟和穗颈瘟的抗性均得到显著提高，表明基因聚合是选育稻瘟病抗性品种的有效方法。
위탐구기인 Pi1여 Pi9적도온병항성，급기인취합대감병수체재료적항성개량정황，이 P121、P32작위공체친본，보지계금23B 작위수체친본，통과상규잡교，회교수단，결합분자표기기술（MAS），도입Pi1、Pi9적쌍기인취합재료，연후대차취합재료화분별휴대도온병항성기인 Pi1、Pi9적선형수도재료 P121、P32，보지계금23B 진행도온병온실인공접충감정급대전자연유발감정。결과표명：수체친본금23B 협온6급감병，수경온9급고감，단기인공체친본 P121、P32협온병급균위4급중감，수경온병급분별위3급중항，7급감병，이이금23B 위배경적항성기인취합재료，협온병급2급，체항급수평，수경온병급3급，체중항수평，상교우수체친본화단기인공체친본，쌍기인취합재료대협온화수경온적항성균득도현저제고，표명기인취합시선육도온병항성품충적유효방법。
In order to study the blast resistance of genes Pi1 and Pi9 and the resistance improvement of susceptible rice materials pyramided with anti-blast genes,using the rice materials P121 and P32 ,which carry respectively with genes Pi1 and Pi9,as the donors,and the maintainer lines Jin23B as the recipient, two resistance genes (Pi9 and Pi1)were introgressed into Jin23B by taking routine crossing,molecular marker technique,which contributed to the emergence of polymeric material.Then,the resistance of polymeric material was identified respectively by artificially inoculating rice blast in the greenhouse and being naturally affected in field.Finally,the results show that the polymeric material have done better in resisting leaf and neck blast,comparing to the two donors.Hence,the result can demonstrate that gene pyramiding is an effective approach for breeding rice varieties with durable resistance to blast.The conclusion is based on the experimental data:the susceptibility of the receptor———Jin23B is level 6 in leaf blast,high susceptibility level 9 in neck blast;the donors-P121 (pi1)and P32 (pi9)are both intermediate susceptibility level 4 in leaf blast,respectively intermediate resistant level 3 and high susceptibility level 7 in neck blast;polymeric material is level 2 in leaf blast(reaching a resistance level),level 3 in neck blast (reaching intermediate resistant level).