CAJ | 학술논문

目的：检测子宫内膜样腺癌组织中LRP16的表达,探讨其与人子宫内膜样腺癌的关联及其与E-cadherin的调控关系。方法 RT-PCR法检测子宫内膜样腺癌组织中LRP16 mRNA的表达；免疫组化方法检测子宫内膜样腺癌组织中LRP16和E-cadherin表达部位及表达水平,分析两者之间的关系。结果子宫内膜样腺癌组织中LRP16mRNA的阳性表达率及表达水平均高于正常子宫内膜组织(P<0．05)。子宫内膜样腺癌组织中,LRP16核表达程度与病理学分级相关(P<0．05),而LRP16浆表达与病理学分级无相关性(P>0．05)；LRP16浆表达与手术病理分期呈负相关(P<0．05),而LRP16核表达与手术病理分期无相关性( P>0．05)；LRP16和E-cadherin的表达无相关性( P>0．05)。结论子宫内膜样腺癌组织中LRP16 mRNA的表达明显高于正常子宫内膜组织；且LRP16蛋白产物核表达水平与癌细胞的分化程度呈负相关；LRP16的过表达与子宫内膜样腺癌的发生和发展可能存在重要联系；而LRP16蛋白的表达水平与E-cadherin的表达可能无相关性。
목적：검측자궁내막양선암조직중LRP16적표체,탐토기여인자궁내막양선암적관련급기여E-cadherin적조공관계。방법 RT-PCR법검측자궁내막양선암조직중LRP16 mRNA적표체；면역조화방법검측자궁내막양선암조직중LRP16화E-cadherin표체부위급표체수평,분석량자지간적관계。결과자궁내막양선암조직중LRP16mRNA적양성표체솔급표체수평균고우정상자궁내막조직(P<0．05)。자궁내막양선암조직중,LRP16핵표체정도여병이학분급상관(P<0．05),이LRP16장표체여병이학분급무상관성(P>0．05)；LRP16장표체여수술병리분기정부상관(P<0．05),이LRP16핵표체여수술병리분기무상관성( P>0．05)；LRP16화E-cadherin적표체무상관성( P>0．05)。결론자궁내막양선암조직중LRP16 mRNA적표체명현고우정상자궁내막조직；차LRP16단백산물핵표체수평여암세포적분화정도정부상관；LRP16적과표체여자궁내막양선암적발생화발전가능존재중요련계；이LRP16단백적표체수평여E-cadherin적표체가능무상관성。
Objective Observe the expression of LRP16 in the tissues of endometrial endometrioid adenocarcinoma. In-vestgate the correlation between expression of LRP16 and E-cadherin in endometria cancer Methods RT-PCR was used to exam-ine the expression of LRP16 inendometrial endometrioid adenocarcinoma specimens. The immuno-histochemical method was used to examine the LRP16 protein in the tissue of endometrial endometrioid adenocarcinoma. Results The expression rate and level of LRP16 mRNA in endometrial cancer were higher than in normal tissue (P<0. 05). LRP16 protein was expressed in the nucleus or cytoplasm of endometrial cancer specimens. The levels of LRP16 protein in the nucleus were related in the degrees of differentiation of the cancer cells (P<0. 05). LRP16 expression in the cytoplasm was no related with grade (P>0. 05). There was no relation-ship between LRP16 expression in the nucleue and staging(P>0. 05). LRP16 expression in the cytoplasm was related with staging (P<0. 05). No correlation was found between LRP16 and E-cadherin in cancer of endometrium. Conclusion The expression of LRP16 protein in the endometrial cancer is higher than that in the normal endometrial. The relationship between the degrees of differ-entiation of endometrial cancer cells and the expressions of endometrial protein is negative correlation. The over-expression of LRP16 should be related with the occurrence and development of endometrial cancer. But there was no significant difference between the ex-pression of LRP16 and E-cadherin in endometrial cancer.