CAJ | 학술논문

目的：分析SCCmec相关的psm-mec在临床分离表皮葡萄球菌中的分布和特征,为深入了解其在表皮葡萄球菌中的功能奠定基础。方法收集临床分离并经过全自动微生物鉴定系统准确鉴定的表皮葡萄球菌84株,PCR扩增esp和mecA基因区分耐甲氧西林表皮葡萄球菌( MRSE),PCR扩增psm-mec和fudoh基因确定携带psm-mec菌株,分析其在不同标本来源MRSE的分布,同时扩增mecR1/psm-mec与psm-mec/xylR基因间隔序列,探讨psm-mec与mecR1,xylR基因的连接关系。结果 PCR扩增结果显示,临床分离表皮葡萄球菌中, MRSE为64株,占76．19%。 psm-mec和fudoh扩增结果显示,psm-mec基因阳性MRSE为25株,携带率为39．0%(25/64),且主要分布于血液和痰液来源MRSE。20株甲氧西林敏感表皮葡萄球菌均未检出psm-mec基因。基因间隔序列扩增结果显示,25株携带psm-mec菌株的mecR1/psm-mec均阳性,16株psm-mec/xylR阳性。结论 SCCmec相关的psm-mec广泛分布于MRSE中,psm-mec均与SCCmec基因盒上mecR1基因相连,64%菌株与xylR连接。
목적：분석SCCmec상관적psm-mec재림상분리표피포도구균중적분포화특정,위심입료해기재표피포도구균중적공능전정기출。방법수집림상분리병경과전자동미생물감정계통준학감정적표피포도구균84주,PCR확증esp화mecA기인구분내갑양서림표피포도구균( MRSE),PCR확증psm-mec화fudoh기인학정휴대psm-mec균주,분석기재불동표본래원MRSE적분포,동시확증mecR1/psm-mec여psm-mec/xylR기인간격서렬,탐토psm-mec여mecR1,xylR기인적련접관계。결과 PCR확증결과현시,림상분리표피포도구균중, MRSE위64주,점76．19%。 psm-mec화fudoh확증결과현시,psm-mec기인양성MRSE위25주,휴대솔위39．0%(25/64),차주요분포우혈액화담액래원MRSE。20주갑양서림민감표피포도구균균미검출psm-mec기인。기인간격서렬확증결과현시,25주휴대psm-mec균주적mecR1/psm-mec균양성,16주psm-mec/xylR양성。결론 SCCmec상관적psm-mec엄범분포우MRSE중,psm-mec균여SCCmec기인합상mecR1기인상련,64%균주여xylR련접。
Objective To investigate the distribution and characteristics of SCCmec-associated psm-mec in Staphylococ-cus epidermidis of clinical isolates,and to lay a foundation for further study on its function of psm-mec in Staphylococcus epidermi-dis. Methods 84 strains of Staphylococcus epidermidis were collected and identified by full automation microbiological identifica-tion system. Esp and mecA genes were amplified by PCR to differentiate methicillin-resistant Staphylococcus epidermidis ( MRSE) . Psm-mec and fudoh genes were used to determine psm-mec positive strains,and the distribution of psm-mec in MRSE i-solated from different sample type was analyzed. The regions from mecR1 to psm-mec and from psm-mec to xylR were amplified to explore the relationship between psm-mec and mecR1,xylR. Results PCR results of esp and mecA showed that there were 64 strains of MRSE with a rate of 76. 19%. 25 psm-mec positive strains mainly from blood and sputum were confirmed by the amplifi-cation results of psm-mec and fudoh genes, and the rate of psm-mec in MRSE was 39. 0%( 25/64 ) . And no psm-mec positive strains were found in methicillin-sensitive Staphylococcus epidermidis. In 25 strains of MRSE with psm-mec,all strains were posi-tive to mecR1/psm-mec,and only 16 strains positive to psm-mec/xylR. Conclusion SCCmec-associated psm-mec exists in MRSE of clinical isolates. Psm-mec connects with mecR1 in all strains,but connects with xylR only in 64% strains.