CAJ | 학술논문

以铁皮石斛原球茎为受体，用农杆菌介导1-氨基环丙烷-1-羧酸氧化酶（ ACO）的反义基因对铁皮石斛进行遗传转化，并对影响转化的几个因素进行优化，结果表明：经刀切法处理的原球茎，在 AS 浓度为100μmol/L、农杆菌菌液 OD600值为0.8的条件下侵染30 min，共培养60 h，接种到含有30 mg/L Mer和3.0 mg/L PPT的筛选培养基中，遗传转化效率最高。筛选获得的抗性植株经 GUS组织化学染色和 PCR 鉴定，初步证明 ACO 反义基因已整合到铁皮石斛的基因组中。
이철피석곡원구경위수체，용농간균개도1-안기배병완-1-최산양화매（ ACO）적반의기인대철피석곡진행유전전화，병대영향전화적궤개인소진행우화，결과표명：경도절법처리적원구경，재 AS 농도위100μmol/L、농간균균액 OD600치위0.8적조건하침염30 min，공배양60 h，접충도함유30 mg/L Mer화3.0 mg/L PPT적사선배양기중，유전전화효솔최고。사선획득적항성식주경 GUS조직화학염색화 PCR 감정，초보증명 ACO 반의기인이정합도철피석곡적기인조중。
The ACO antisense gene was transformed into the protocorms of Dendrobiun officinale by Agrobacte-rium mediation. To optimize the transformation efficiency,several related factors were studied in this paper. The re-sults showed that,after infected for 30 min at 100 μmol/L of AS,0. 8 of OD600 and 60 h of incubation in darkness, the cut protocorms gained the highest transformation rate in the medium of 30 mg/L Mer and 3. 0 mg/L PPT. The selected plants were tested by PCR and GUS histochemical staining,indicating that the ACO antisense gene had been successfully integrated into D. officinale.