CAJ | 학술논문

目的：评价七氟醚预处理对老龄大鼠术后认知功能障碍的影响及海马区炎性细胞因子含量在其中的作用。方法健康成年雄性SD大鼠138只，18个月龄，体质量400~450g，采用随机数字表法，将其分为3组（n=46）。对照组（C组）：仅戊巴比妥钠麻醉，不进行手术；手术组（O组）：戊巴比妥钠麻醉后行剖腹探查术，手术时间30min；七氟醚预处理组（Sev组）：吸入2.4%七氟醚30min，然后吸入空气30min后处理同O组。于术前30min及术后第1、3、5、7天时，行Morris水迷宫实验，记录逃避潜伏期、平台象限停留时间和穿越原平台次数；于术前30min及术后第1、7天时，取海马组织，采用ELISA法测定海马组织TNF-α、IL-1β及IL-6含量；采用流式细胞术测定海马神经元凋亡率、胞质钙离子浓度（[Ca2+]i），并于术毕即刻，采集动脉血样，行血气分析及记录体温。结果与C组比较，O组术后各时点和Sev组术后第1、3、5天时逃避潜伏期延长、平台象限停留时间缩短和穿越原平台次数减少，O组术后第1、7天和Sev组术后第1天时海马TNF-α、IL-1β及IL-6含量升高，海马神经元凋亡率、胞质钙离子浓度（[Ca2+]i）升高（P<0.05）；与O组比较，Sev组术后各时点逃避潜伏期缩短、平台象限停留时间延长和穿越原平台次数增多，海马TNF-α、IL-1β及IL-6含量降低，海马神经元凋亡率、胞质钙离子浓度（[Ca2+]i）降低（P<0.05）。结论2.4%七氟醚预处理可减轻老龄大鼠术后认知功能障碍，其机制与降低海马区TNF-α、IL-1β及IL-6含量有关。
목적：평개칠불미예처리대노령대서술후인지공능장애적영향급해마구염성세포인자함량재기중적작용。방법건강성년웅성SD대서138지，18개월령，체질량400~450g，채용수궤수자표법，장기분위3조（n=46）。대조조（C조）：부무파비타납마취，불진행수술；수술조（O조）：무파비타납마취후행부복탐사술，수술시간30min；칠불미예처리조（Sev조）：흡입2.4%칠불미30min，연후흡입공기30min후처리동O조。우술전30min급술후제1、3、5、7천시，행Morris수미궁실험，기록도피잠복기、평태상한정류시간화천월원평태차수；우술전30min급술후제1、7천시，취해마조직，채용ELISA법측정해마조직TNF-α、IL-1β급IL-6함량；채용류식세포술측정해마신경원조망솔、포질개리자농도（[Ca2+]i），병우술필즉각，채집동맥혈양，행혈기분석급기록체온。결과여C조비교，O조술후각시점화Sev조술후제1、3、5천시도피잠복기연장、평태상한정류시간축단화천월원평태차수감소，O조술후제1、7천화Sev조술후제1천시해마TNF-α、IL-1β급IL-6함량승고，해마신경원조망솔、포질개리자농도（[Ca2+]i）승고（P<0.05）；여O조비교，Sev조술후각시점도피잠복기축단、평태상한정류시간연장화천월원평태차수증다，해마TNF-α、IL-1β급IL-6함량강저，해마신경원조망솔、포질개리자농도（[Ca2+]i）강저（P<0.05）。결론2.4%칠불미예처리가감경노령대서술후인지공능장애，기궤제여강저해마구TNF-α、IL-1β급IL-6함량유관。
?Objective?To?investigate?the?effects?of?preconditioning?with?sevoflurane?inhalation?on?postoperative?cognition?disorders?of?aged?rats?and?the?role?of?inflammatory?factor?levels?in?the?hippocampus.?Methods?One?hundred?and?thirty-eight?healthy?male?SD?rats,?aged?18?months,?weighing?400-450?g?were?randomly?devided?into?3?groups?(n=46?each).?Control?group?(group?C)?were?only?anesthetized?with?pentobarbital?and?not?underwent?operation;?operation?group?(group?O)?were?anesthetized?with?pentobarbital?and?underwent?30?min?of?exploratory?laparotomy;?sevoflurane?preconditioning?group?(group?Sev)?were?inhaled?2.4%?sevoflurane?30?min,then?treat?like?group?O?following?by?inhaled?room?air?30?min.?The?escape?latency,?the?time?of?staying?at?the?original?platform?quadrant?and?frequency?of?crossing?the?original?platform?was?assessed?using?Morris?water?maze?on?30?min?before?operation?and?1st,?3rd,?5th,?7th?days?after?operation.?The?brain?tissues?were?taken?for?detecting?the?levels?of?inflammatory?factor,the?apoptotic?rate?and?intracellular?[Ca2+]i?of?hippocampal?neuronal?at?30?min?before?operation?and?1st,?7th?days?after?operation.?And?immediately?after?surgery?arterial?blood?samples?were?taken?for?blood?gas?analysis,?at?the?same?time?points?body?temperature?was?recorded.?Results?Compared?with?group?C,?the?escape?latency?was?prolonged,?the?time?of?staying?at?the?original?platform?quadrant?and?frequency?of?crossing?the?original?platform?were?decreased at all time points in group O and at the 1st, 3rd, 5th day after operation in group Sev; the levels of TNF-α,IL-1β,IL-6 of the hippocampus and?the?apoptotic?rate?and?intracellular?[Ca2+]i?of?hippocampal?neuronal?were?increased?at?the?1st,?7th?day?after?operation?in?group?O?and?at?the?1st?day?in?group?Sev(P<0.05),?compared?with?group?O,?the?escape?latency?was?shorten,?the?time?of?staying?at?the?original?platform?quadrant?and?frequency?of?crossing the original platform were increased, the levels of TNF-α, IL-1β, IL-6 and the apoptotic rate and intracellular [Ca2+]i?of?hippocampal?neuronal?were?decreased?at?all?time?points?after?operation?in?group?Sev(P<0.05).?Conclusion?Sevoflurane?preconditioning?can?improve?postoperative?cognitive?function of aged rats, which may be related to decrease the levels of TNF-α,?IL-1β and IL-6 and regulation of imbalance of calcium homeostasis and reduction?the?apoptotic?rate?of?hippocampal?neuronal.