CAJ | 학술논문

目的：探讨血管紧张素转换酶（ACE）基因插入/缺失（insertion/deletion，I/D）多态性与冠状动脉粥样硬化性心脏病（冠心病）及冠状动脉（冠脉）病变支数间的关系。方法选取住院患者332例，其籍贯均为河北唐山地区，行冠脉造影术检查判定其是否患有冠心病及其冠脉病变支数。根据造影结果分为冠心病组（至少有一支冠脉主支血管狭窄≥50%）233例（稳定型心绞痛亚组150例、急性冠脉综合征组亚组83例）和对照组99例（任一冠脉主支血管狭窄＜50%）。应用聚合酶链式反应技术检测患者ACE基因I/D多态性，包括II型（插入型纯合子）、ID型（插入与缺失型杂合子）、DD型（缺失型纯合子）三组，并分析不同组别间I/D多态性差异。结果冠心病组及急性冠脉综合征亚组ACE基因DD型和D等位基因频率显著高于对照组（P＜0.05）。稳定型心绞痛组与对照组比较DD基因型和D等位基因频率间并无显著差异（P＞0.05）。不同冠脉病变支数在ACE基因型间差异无统计学意义（P＞0.05）。结论唐山地区汉族人群ACE基因I/D多态性中DD型和D等位基因发生率冠心病及急性冠脉综合征患者中增高，但与冠脉病变支数无关。
목적：탐토혈관긴장소전환매（ACE）기인삽입/결실（insertion/deletion，I/D）다태성여관상동맥죽양경화성심장병（관심병）급관상동맥（관맥）병변지수간적관계。방법선취주원환자332례，기적관균위하북당산지구，행관맥조영술검사판정기시부환유관심병급기관맥병변지수。근거조영결과분위관심병조（지소유일지관맥주지혈관협착≥50%）233례（은정형심교통아조150례、급성관맥종합정조아조83례）화대조조99례（임일관맥주지혈관협착＜50%）。응용취합매련식반응기술검측환자ACE기인I/D다태성，포괄II형（삽입형순합자）、ID형（삽입여결실형잡합자）、DD형（결실형순합자）삼조，병분석불동조별간I/D다태성차이。결과관심병조급급성관맥종합정아조ACE기인DD형화D등위기인빈솔현저고우대조조（P＜0.05）。은정형심교통조여대조조비교DD기인형화D등위기인빈솔간병무현저차이（P＞0.05）。불동관맥병변지수재ACE기인형간차이무통계학의의（P＞0.05）。결론당산지구한족인군ACE기인I/D다태성중DD형화D등위기인발생솔관심병급급성관맥종합정환자중증고，단여관맥병변지수무관。
Objective To discuss the relationship between gene insertion/deletion (I/D) polymorphism of angiotensin-converting enzyme (ACE) and coronary heart disease (CHD) or number of diseased coronary vessels. Methods The in-hospital patients (n=332, all from Tangshan region of Hebei Province) were chosen and given coronary angiography (CAG) for adjusting whether or not they suffered from CHD and number of diseased coronary vessels. According to the outcomes of CAG, the patients were divided into CHD group [at least 1 coronary major branch being stenosis≥50%,n=233, among them 150 were in stable angina pectoris (SAP) subgroup and 83 in acute coronary syndrome (ACS) subgroup] and control group (n=99, anyone coronary major branch being stenosis<50%). I/D polymorphism of ACE was detected by using polymerase chain reaction (PCR), including type II (type of insertion homozygous), type ID (type of insertion and deletion heterozygous) and type DD (type of deletion homozygous), and the difference in I/D polymorphism were compared among all groups.Results The frequency of type DD and D allele was significantly higher in CHD group and ACS subgroup than that in control group (P<0.05), and that had no significant difference between SAP subgroup and control group (P>0.05). The difference in number of diseased coronary vessels had no statistical significance among different ACE gene types (P>0.05).Conclusion In population of Han nationality in Tangshan region, type DD and D allele of ACE I/D polymorphism increase in patients with CHD and ACS, which, however, is not correlated to number of diseased coronary vessels.