华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2015年
2期
100-103
,共4页
单丽萍%王昌禄%李贞景%陈勉华%李风娟
單麗萍%王昌祿%李貞景%陳勉華%李風娟
단려평%왕창록%리정경%진면화%리풍연
链霉菌 TD-1%灰霉菌%防御酶%生物防治
鏈黴菌 TD-1%灰黴菌%防禦酶%生物防治
련매균 TD-1%회매균%방어매%생물방치
Streptonyces alboflavus TD-1%Botrytis cinerea%Defensive enzymes%Biological control
采用体外抑菌活性和盆栽试验测定方法,研究了白黄链霉菌 TD-1发酵液对番茄灰霉病菌的抑制效果及植株体内防御酶活性的影响,旨在为番茄灰霉病的生物防治提供参考。结果表明:TD-1菌株发酵液对灰霉病菌的菌丝生长和孢子萌发抑制率分别为98.1%和98.3%;TD-1发酵液在番茄植株上对灰霉病的防治效果为76.98%。喷洒TD-1发酵液24 h后接种灰霉菌孢子悬浮液,番茄叶片中苯丙氨酸解氨酶( PAL)、多酚氧化酶( PPO)、过氧化物酶(POD)和超氧化物歧化酶(SOD)的活力均有所提高,分别在处理后第4,3,2,3天达到最高。
採用體外抑菌活性和盆栽試驗測定方法,研究瞭白黃鏈黴菌 TD-1髮酵液對番茄灰黴病菌的抑製效果及植株體內防禦酶活性的影響,旨在為番茄灰黴病的生物防治提供參攷。結果錶明:TD-1菌株髮酵液對灰黴病菌的菌絲生長和孢子萌髮抑製率分彆為98.1%和98.3%;TD-1髮酵液在番茄植株上對灰黴病的防治效果為76.98%。噴灑TD-1髮酵液24 h後接種灰黴菌孢子懸浮液,番茄葉片中苯丙氨痠解氨酶( PAL)、多酚氧化酶( PPO)、過氧化物酶(POD)和超氧化物歧化酶(SOD)的活力均有所提高,分彆在處理後第4,3,2,3天達到最高。
채용체외억균활성화분재시험측정방법,연구료백황련매균 TD-1발효액대번가회매병균적억제효과급식주체내방어매활성적영향,지재위번가회매병적생물방치제공삼고。결과표명:TD-1균주발효액대회매병균적균사생장화포자맹발억제솔분별위98.1%화98.3%;TD-1발효액재번가식주상대회매병적방치효과위76.98%。분쇄TD-1발효액24 h후접충회매균포자현부액,번가협편중분병안산해안매( PAL)、다분양화매( PPO)、과양화물매(POD)화초양화물기화매(SOD)적활력균유소제고,분별재처리후제4,3,2,3천체도최고。
The inhibitory effect of Streptonyces alboflavus TD-1 against Botrytis cinerea and its effect on defensive enzymes activity in vitro antibacteral activity and pot experiment were studied in order to provide reference for biologi-cal control of grey mould. The results showed that inhibition rate of fermentation broth of the strain TD-1 on mycelium growth and spore germination of B. cinerea were 98. 1% and 98. 3% respectively. The control efficiency of TD-1 fer-mentation broth against B. cinerea in tomato plants was 76. 98% in pot experiment. The activity of PAL,PPO,POD and SOD of tomato leaves increased after spraying fermentation broth of the strain TD-1 and spore suspension of B. cine-rea. They reached maximum in the fourth day,the third day,the second day and the third day respectively.