CAJ | 학술논문

为优选金果榄总黄酮的最佳提取工艺并对其黄酮的体外抗氧化活性进行评价，采用微波法对金果榄总黄酮进行提取，采用正交试验设计，考察乙醇质量浓度、微波功率、固液比、提取时间对金果榄总黄酮提取率的影响，用芦丁比较其对羟自由基、超氧阴离子的清除能力。结果表明：微波提取最佳的工艺条件为乙醇质量浓度60％、固液比为1∶20、提取时间8 min、微波功率640 W；抗氧化性评价表明，总黄酮对羟自由基、超氧阴离子的清除率为50％时，其浓度分别为2．766 mg／mL 和4．571 mg／mL。最优条件下的总黄酮得率为11．384 mg／g，金果榄总黄酮对羟自由基、超氧阴离子均具有不同程度的清除能力。
위우선금과람총황동적최가제취공예병대기황동적체외항양화활성진행평개，채용미파법대금과람총황동진행제취，채용정교시험설계，고찰을순질량농도、미파공솔、고액비、제취시간대금과람총황동제취솔적영향，용호정비교기대간자유기、초양음리자적청제능력。결과표명：미파제취최가적공예조건위을순질량농도60％、고액비위1∶20、제취시간8 min、미파공솔640 W；항양화성평개표명，총황동대간자유기、초양음리자적청제솔위50％시，기농도분별위2．766 mg／mL 화4．571 mg／mL。최우조건하적총황동득솔위11．384 mg／g，금과람총황동대간자유기、초양음리자균구유불동정도적청제능력。
The effect of ethanol mass concentration,microwave power,solid-liquid ratio and time on extraction rate of total flavones extracted from R.tinosporae by using microwave method was studied by the orthogonal test design,and scavenging activity of the extracted flavones to hydroxyl radical and superoxide anion was analyzed to select the optimum extraction technology of total flavones from R.tinosporae and evaluate in vitro antioxidant activity of the extracted flavones.The results showed that the optimum technological conditions are 60% ethanol mass concentration,1:20 of solid-liquid ratio,640W for 8 min and the scavenging rates of total flavones with 2.766 mg/mL and 4.571 mg/mL concentration to hydroxyl radical and superoxide anion are 50%.Total flavones extracted from R.tinosporae can reach 11.384 mg/gunder the optimum extraction conditions.Total flavones extracted from R.tinosporae has scavenging activities with different degree to hydroxyl radical and superoxide anion.