CAJ | 학술논문

目的：了解贵阳地区丙型肝炎患者的HCV基因分型情况，探讨不同 HCV基因分型患者外周血辅助性T细胞因子的差异。方法收集2011年10月至2013年7月在贵阳市第五人民医院住院和门诊的198例慢性丙型肝炎患者样本，分别采用直接测序法检测丙型肝炎基因型，ELISA法检测血清中细胞因子白介素2（IL‐2）、细胞因子白介素8（IL‐8）、细胞因子白介素10（IL‐10）和肿瘤坏死因子α（TFN‐α）的含量。结果198份丙型肝炎患者HCV基因分型结果显示，1a型4例（2．02％），1b型71例（35．86％），2a型9例（4．55％），3a型29例（14．65％），3b型47例（23．74％），6a型37例（18．69％），6d型1例（0．50％）；慢性丙型肝炎患者血清IL‐8与正常对照组比较差异无统计学意义，而慢性丙型肝炎患者血清IL‐2、IL‐10、T N F‐α与正常对照组比较差异有统计学意义；慢性丙型肝炎患者IL‐2与TNF‐α在各基因型间比较差异无统计学意义，lb型患者血清IL‐8与IL‐10含量明显高于3a、3b、6a型患者，差异有统计学意义。结论贵州地区 HCV 基因型分布呈现多样性。贵州地区丙型肝炎1b型患者细胞因子水平失衡可能是导致其慢性化率高的主要因素。
목적：료해귀양지구병형간염환자적HCV기인분형정황，탐토불동 HCV기인분형환자외주혈보조성T세포인자적차이。방법수집2011년10월지2013년7월재귀양시제오인민의원주원화문진적198례만성병형간염환자양본，분별채용직접측서법검측병형간염기인형，ELISA법검측혈청중세포인자백개소2（IL‐2）、세포인자백개소8（IL‐8）、세포인자백개소10（IL‐10）화종류배사인자α（TFN‐α）적함량。결과198빈병형간염환자HCV기인분형결과현시，1a형4례（2．02％），1b형71례（35．86％），2a형9례（4．55％），3a형29례（14．65％），3b형47례（23．74％），6a형37례（18．69％），6d형1례（0．50％）；만성병형간염환자혈청IL‐8여정상대조조비교차이무통계학의의，이만성병형간염환자혈청IL‐2、IL‐10、T N F‐α여정상대조조비교차이유통계학의의；만성병형간염환자IL‐2여TNF‐α재각기인형간비교차이무통계학의의，lb형환자혈청IL‐8여IL‐10함량명현고우3a、3b、6a형환자，차이유통계학의의。결론귀주지구 HCV 기인형분포정현다양성。귀주지구병형간염1b형환자세포인자수평실형가능시도치기만성화솔고적주요인소。
Objective To know the HCV genetic typing of patients of hepatitis c in Guiyang district and study the difference of peripheral blood helper T cell factors of patients of HCV genetic type .Method Collected the blood sam‐ples of 198 patients of chronic hepatitis c seeking for medical treatment in inpatient department and clinic of Guiyang fifth People’s Hospital from October ,2011 to July ,2013 ,and direct sequencing method was applied to detect the genetic type of hepatitis C and ELISA method to detect the contents of cell factor interleukin 2 (IL‐2) in serum ,cell factor interleukin8 (IL‐8) ,cell factor interleukin10 (IL‐10) and tumor necrosis factorα(TFN‐α) .Result The result of HCV genetic type of 198 patients of hepatitis c shows that type 1a ,4 cases (2 .02% );type 1b ,71 cases (35 .86% );type 2a ,9 cases (4 .55% );type 3a ,29 cases (14 .65% );type 3b ,47 cases (23 .74% );type 6a ,37 ca‐ses (18 .69% );type 6d ,1 case (0 .50% ) .The comparison between chronic hepatitis c patients of serum IL‐8 and normal control group shows no statistical significance while the comparison between chronic hepatitis c patients of serum IL‐8 ,IL‐10 and TNF‐αwith normal control group shows statistical significance .Chronic hepatitis c patients of IL‐2 and TNF‐αshown that there were no statistical significantly differences in the comparison of genetic types . The type 1b patients had significantly higher IL‐8 and IL‐10 contents than type 3a ,3b and 6a patients ,and the com‐parison shown there were statistical significantly differences .Conclusion HCV genetic type distribution in Guizhou Province presents diversity .The unbalance of cell factor level of type 1b patients in Guizhou province may be the main factor leading to higher chronic rate .