CAJ | 학술논문

目的：观察不同浓度氯胺酮（Ketamine ，KET ）家兔关节腔内注射后软骨细胞Bcl‐2及Bax表达的影响。方法通过管型石膏伸直位制动法造模成功后，随机分为模型组、氯胺酮60组、氯胺酮100组、氯胺酮200组，其中模型组、氯胺酮60组、氯胺酮100组、氯胺酮200组分别给予0．4 mL的生理盐水、KET（60μmol／L）、KET（100μmol／L）、KET（200μmol／L）关节腔内注射，每周2次，共进行4周，最后一次注射后1周处死动物。留取膝关节软骨标本作相应处理后，利用免疫组化法观察关节软骨中Bcl‐2及Bax的表达情况。结果与模型组比较，注射氯胺酮的软骨细胞促凋亡基因Bcl‐2及Bax均改变（P＜0．05），其中氯胺酮200组的抗凋亡效果最为显著（P＜0．05）。结论氯胺酮可以抑制骨关节炎诱导的软骨细胞凋亡。
목적：관찰불동농도록알동（Ketamine ，KET ）가토관절강내주사후연골세포Bcl‐2급Bax표체적영향。방법통과관형석고신직위제동법조모성공후，수궤분위모형조、록알동60조、록알동100조、록알동200조，기중모형조、록알동60조、록알동100조、록알동200조분별급여0．4 mL적생리염수、KET（60μmol／L）、KET（100μmol／L）、KET（200μmol／L）관절강내주사，매주2차，공진행4주，최후일차주사후1주처사동물。류취슬관절연골표본작상응처리후，이용면역조화법관찰관절연골중Bcl‐2급Bax적표체정황。결과여모형조비교，주사록알동적연골세포촉조망기인Bcl‐2급Bax균개변（P＜0．05），기중록알동200조적항조망효과최위현저（P＜0．05）。결론록알동가이억제골관절염유도적연골세포조망。
Objective To explore the different concentrations of after the Bcl‐2 ,and the influence of Bax expres‐sion .Method The rest of rabbits with left knee immobilized in extension position to establish osteoarthritis models , then ,divided the models into model group ,ketamine60 group ,ketamine100 group and ketamine200 group in ran‐dom .They were received 0 .4 ml physiologic saline ,KET of 60μmol/L ,KET of 100μmol/L ,KET of 200μmol/L intra‐articular respectively ,two times a week for 4 weeks ,and sacrificed one week after last injection .To return af‐ter knee joint cartilage specimens corresponding processing ,using immunohistochemical method to observe the Bcl‐2 in the articular cartilage and the expression of bax .Result Compared with model group ,the injection of ketamine and promote cartilage cells apoptosis gene Bcl‐2 and Bax genes are changed (P<0 .05) .ketamine200 group of antiapop‐totic effect is the most significant (P<0 .05) .Conclusion Ketamine can suppress the cartilage cell apoptosis induced by osteoarthritis .