CAJ | 학술논문

目的：观察槐耳颗粒在体外对结肠癌SW480细胞侵袭转移的影响，并初步探讨其机制。方法 SubG1法确定槐耳颗粒促进结肠癌SW480细胞凋亡的最佳作用浓度和时间，通过划痕愈合实验和Transwell侵袭实验观察槐耳颗粒对结肠癌SW480细胞侵袭转移的影响。 Western blot法和 Real-Time PCR (荧光定量 PCR )法检测 E-cadherin、twist、snail、vimentin在蛋白和mRNA水平的变化。结果槐耳颗粒3.0 g·L-1处理SW480细胞36 h后凋亡最明显。槐耳颗粒3.0 g·L-1处理SW480细胞36 h后，划痕愈合实验相对迁移率为(31.36±2.39)%，而对照组36 h后相对迁移率则为(61.11±1.09)%(P<0.01)。 Transwell侵袭实验证实槐耳颗粒3.0 g·L-1处理SW480细胞36 h后每个视野穿透细胞数为(129±12)个，而对照组为(354±20)个(P<0.01)。槐耳颗粒3.0 g·L-1处理SW480细胞36 h后E-cadherin 在蛋白和mRNA水平表达均升高，而twist、snail和vimentin表达均降低。结论槐耳颗粒在体外能够通过抑制上皮-间质转化有效抑制结肠癌SW480细胞的侵袭转移。
목적：관찰괴이과립재체외대결장암SW480세포침습전이적영향，병초보탐토기궤제。방법 SubG1법학정괴이과립촉진결장암SW480세포조망적최가작용농도화시간，통과화흔유합실험화Transwell침습실험관찰괴이과립대결장암SW480세포침습전이적영향。 Western blot법화 Real-Time PCR (형광정량 PCR )법검측 E-cadherin、twist、snail、vimentin재단백화mRNA수평적변화。결과괴이과립3.0 g·L-1처리SW480세포36 h후조망최명현。괴이과립3.0 g·L-1처리SW480세포36 h후，화흔유합실험상대천이솔위(31.36±2.39)%，이대조조36 h후상대천이솔칙위(61.11±1.09)%(P<0.01)。 Transwell침습실험증실괴이과립3.0 g·L-1처리SW480세포36 h후매개시야천투세포수위(129±12)개，이대조조위(354±20)개(P<0.01)。괴이과립3.0 g·L-1처리SW480세포36 h후E-cadherin 재단백화mRNA수평표체균승고，이twist、snail화vimentin표체균강저。결론괴이과립재체외능구통과억제상피-간질전화유효억제결장암SW480세포적침습전이。
Objective To investigate the effects of huaier granules on invasion and metastasis of colorectal cancer SW480 cells in vitro, and explore the basic mechanism. Methods The appropriate concentration and duration of huaier granules promoting SW480 cell apoptosis were determined by SubG1 method. Wound healing assay and transwell assay were used to observe the effect of huaier granules on SW480 cell invasion and metastasis. The changes of E-cadherin, twist, snail and vimentin at protein and mRNA levels were examined by Western blotting and Real-Time PCR. Results After treatment with huaier granules at 3. 0 g·L-1 for 36 h, apoptosis of SW480 cells was most significant, and wound healing assay revealed that relative mobility was (31. 36±2. 39)%, compared with (61. 11±1. 09)% in control group (P<0. 01). Number of invaded cells per field of view was (129±12) in treatment group and (354±20) in control group (P<0. 01). After treatment with huaier granules at 3. 0 g·L-1 for 36 h, protein and mRNA levels of E-cadherin were increased, while those of twist, snail and vimentin were decreased. Conclusion Huaier granules can inhibit invasion and metastasis of colorectal cancer SW480 cells in vitro through effectively depressing epithelial-mesenchymal transition.