延边大学学报（自然科学版）
延邊大學學報（自然科學版）
연변대학학보（자연과학판）
JOURNAL OF YANBIAN UNIVERSITY(NATURAL SCIENCE EDITION)
2015年
1期
79-84
,共6页
党参%稀有人参皂苷%内生菌%生物转化
黨參%稀有人參皂苷%內生菌%生物轉化
당삼%희유인삼조감%내생균%생물전화
Codonopsis pilosula%minor ginsenosides%endophytes%biotransformation
利用组织分离法从党参中分离得到32种内生菌，其中菌株 D19转化人参主皂苷为稀有皂苷的活性最强．在最佳发酵条件（水培养基，pH 5．0）下，稀有人参皂苷 F2、C-K 和 Rh1的最高产率分别为30％、17％和8％．根据单体皂苷转化实验确定了稀有人参皂苷的来源和人参皂苷 Rb1的转化路径，并通过16S rDNA 序列分析，鉴定内生菌 D19为藤黄色杆菌属（Dyella）．
利用組織分離法從黨參中分離得到32種內生菌，其中菌株 D19轉化人參主皂苷為稀有皂苷的活性最彊．在最佳髮酵條件（水培養基，pH 5．0）下，稀有人參皂苷 F2、C-K 和 Rh1的最高產率分彆為30％、17％和8％．根據單體皂苷轉化實驗確定瞭稀有人參皂苷的來源和人參皂苷 Rb1的轉化路徑，併通過16S rDNA 序列分析，鑒定內生菌 D19為籐黃色桿菌屬（Dyella）．
이용조직분리법종당삼중분리득도32충내생균，기중균주 D19전화인삼주조감위희유조감적활성최강．재최가발효조건（수배양기，pH 5．0）하，희유인삼조감 F2、C-K 화 Rh1적최고산솔분별위30％、17％화8％．근거단체조감전화실험학정료희유인삼조감적래원화인삼조감 Rb1적전화로경，병통과16S rDNA 서렬분석，감정내생균 D19위등황색간균속（Dyella）．
A total of 32 kinds of endophytes form Codonopsis pilosula were isolated through the method of organizational separation.Among these endophytes,strains D19 showed the strongest activities to convert major ginsenosides to minor ginsenosides.When in the medium of H2 O,pH of 5.0,the maximum yield of minor ginsenosides F2,C-K and Rh1 reached to 30%,17% and 8%,respectively.The source of minor ginsenosides and the transformation pathway of ginsenoside Rb1 were confirmed according to the saponin mon-omer conversion experiments.Phylogenetic analysis based on 16S rDNA sequences indicated that the strain D19 belongs to the genus Dyella.
