CAJ | 학술논문

目的：：证实抗表皮生长因子受体单克隆抗体耦联中空金纳米球( anti-EG-FR/HGNs)增加宫颈癌细胞对HGNs的选择性摄取,并增强宫颈癌细胞的放射敏感性。方法：电化学置换法制备HGNs,透射电子显微镜( TEM)观察HGNs的尺寸和形态,电感耦合等离子体质谱( ICP-MS)检测细胞内HGNs摄取量,CCK-8法进行细胞毒性检测,流式细胞术( FCM)检测细胞周期,Western blot法检测Bcl-2、Bax、Bad、active caspase-3等凋亡相关蛋白的表达。结果：HGNs平均直径(54.6±7.11)nm,壁厚(5.01±2.23)nm。 Anti-EGFR/HGNs与细胞共同孵育可致细胞内HGNs摄取量显著增加, anti-EGFR/HGNs联合照射亦表现出显著细胞毒性。 Anti-EGFR/HGNs使处于G2/M期的宫颈癌细胞比率增加,并通过下调Bcl-2表达及上调Bax、Bad、caspase-3表达促进细胞凋亡。结论：anti-EGFR/HGNs能增加宫颈癌细胞对HGNs的摄取,并增强兆伏高能照射对细胞的毒性,提高细胞的放射敏感性。
목적：：증실항표피생장인자수체단극륭항체우련중공금납미구( anti-EG-FR/HGNs)증가궁경암세포대HGNs적선택성섭취,병증강궁경암세포적방사민감성。방법：전화학치환법제비HGNs,투사전자현미경( TEM)관찰HGNs적척촌화형태,전감우합등리자체질보( ICP-MS)검측세포내HGNs섭취량,CCK-8법진행세포독성검측,류식세포술( FCM)검측세포주기,Western blot법검측Bcl-2、Bax、Bad、active caspase-3등조망상관단백적표체。결과：HGNs평균직경(54.6±7.11)nm,벽후(5.01±2.23)nm。 Anti-EGFR/HGNs여세포공동부육가치세포내HGNs섭취량현저증가, anti-EGFR/HGNs연합조사역표현출현저세포독성。 Anti-EGFR/HGNs사처우G2/M기적궁경암세포비솔증가,병통과하조Bcl-2표체급상조Bax、Bad、caspase-3표체촉진세포조망。결론：anti-EGFR/HGNs능증가궁경암세포대HGNs적섭취,병증강조복고능조사대세포적독성,제고세포적방사민감성。
Objective:To confirm that the anti-epidermal growth factor receptor ( EG-FR) monoclonal antibodies conjugated hollow gold nanospheres ( anti-EGFR/HGNs) can be se-lectively uptook by cervical cancer cells and induce its apoptosis when combined with radiother-apy as a result enhancing radiosensitivity of cervical cancer cells. Methods:The HGNs with a mean diameter of ( 54 . 6 ± 7 . 11 ) nm and wall thickness of ( 5 . 01 ± 2 . 23 ) nm were viewed by transmission electron microscopy( TEM) . Cell uptake was assayed by inductively coupled plas-ma mass spectroscopy( ICP-MS) . The cytotoxicity on CaSki cells,which were used in our exper-iment,was assessed by CCK-8 assay. Cell cycle were examined by a propidium iodide( PI) kit with flow cytometry(FCM). The expression of several critical apoptosis-related proteins,inclu-ding Bcl-2,Bax,Bad,active caspase-3,was tested by Western blot analysis. Results:Cells trea-ted by anti-EGFR/HGNs showed an obvious increase in nanoparticles uptake compared to na-ked HGNs. Anti-EGFR/HGNs combined with radiation resulted in a significant growth inhibi-tion,compared with radiation combined with naked HGNs. Anti-EGFR/HGNs remarkably in-creased the ratio of CaSki cells in the G2/M phase and induced more apoptosis by an obvious deregulation of Bcl-2 and upregulation of Bax,Bad,caspase-3 when combined with radiation. Conclusions:Anti-EGFR/HGNs can increase the targeted uptake of HGNs by CaSki cells and enhance radio-cytotoxic targeting of cervical cancer at megavoltage radiation energies.