烟草科技
煙草科技
연초과기
TOBACCO SCIENCE & TECHNOLOGY
2015年
4期
7-12
,共6页
朱奎正%彭耀东%陈祝%张清莉%徐庆凯%王文杰%何宽信%陈学平
硃奎正%彭耀東%陳祝%張清莉%徐慶凱%王文傑%何寬信%陳學平
주규정%팽요동%진축%장청리%서경개%왕문걸%하관신%진학평
β-氨基丁酸(BABA)%烟草%铜胁迫%活性氧%基因表达
β-氨基丁痠(BABA)%煙草%銅脅迫%活性氧%基因錶達
β-안기정산(BABA)%연초%동협박%활성양%기인표체
β-aminobutyric acid%Tobacco%Copper stress%Reactive oxygen species (ROS)%Gene expression
为了有效缓解铜胁迫对烟草的危害,设置了不同浓度β-氨基丁酸（BABA）处理对铜胁迫下烟草幼苗生长、活性氧代谢的影响以及相关铜离子运输基因的表达试验。结果表明：在100μmol/L CuSO4胁迫下，烟草生长受到抑制，与未经BABA处理的烟草幼苗相比，经过BABA处理的烟草幼苗根长和鲜质量呈上升趋势。0.5 mmol/L CuSO4胁迫下，0.2和0.5 mmol/L BABA处理降低了铜胁迫下烟草幼苗体内丙二醛含量（MDA，μmol/g）和过氧化氢含量（H2O2， mol/g），并且提高了抗氧化酶（超氧化物歧化酶SOD,过氧化物酶POD和抗坏血酸过氧化物酶APX）活性。同时调节烟草体内二价金属离子运输的NtNRAMP, NtYSL以及NtPDR基因的表达量显著提高。因此，BABA通过调节烟草体内抗氧化系统和相关金属离子运输基因的表达来缓解铜胁迫对烟草生长的影响。
為瞭有效緩解銅脅迫對煙草的危害,設置瞭不同濃度β-氨基丁痠（BABA）處理對銅脅迫下煙草幼苗生長、活性氧代謝的影響以及相關銅離子運輸基因的錶達試驗。結果錶明：在100μmol/L CuSO4脅迫下，煙草生長受到抑製，與未經BABA處理的煙草幼苗相比，經過BABA處理的煙草幼苗根長和鮮質量呈上升趨勢。0.5 mmol/L CuSO4脅迫下，0.2和0.5 mmol/L BABA處理降低瞭銅脅迫下煙草幼苗體內丙二醛含量（MDA，μmol/g）和過氧化氫含量（H2O2， mol/g），併且提高瞭抗氧化酶（超氧化物歧化酶SOD,過氧化物酶POD和抗壞血痠過氧化物酶APX）活性。同時調節煙草體內二價金屬離子運輸的NtNRAMP, NtYSL以及NtPDR基因的錶達量顯著提高。因此，BABA通過調節煙草體內抗氧化繫統和相關金屬離子運輸基因的錶達來緩解銅脅迫對煙草生長的影響。
위료유효완해동협박대연초적위해,설치료불동농도β-안기정산（BABA）처리대동협박하연초유묘생장、활성양대사적영향이급상관동리자운수기인적표체시험。결과표명：재100μmol/L CuSO4협박하，연초생장수도억제，여미경BABA처리적연초유묘상비，경과BABA처리적연초유묘근장화선질량정상승추세。0.5 mmol/L CuSO4협박하，0.2화0.5 mmol/L BABA처리강저료동협박하연초유묘체내병이철함량（MDA，μmol/g）화과양화경함량（H2O2， mol/g），병차제고료항양화매（초양화물기화매SOD,과양화물매POD화항배혈산과양화물매APX）활성。동시조절연초체내이개금속리자운수적NtNRAMP, NtYSL이급NtPDR기인적표체량현저제고。인차，BABA통과조절연초체내항양화계통화상관금속리자운수기인적표체래완해동협박대연초생장적영향。
In order to alleviate the damage of copper stress on tobacco seedlings, the effects ofβ-aminobutyric acid (BABA) at different concentrations on the growth of seedlings, metabolism of reactive oxygen species (ROS) and copper ion transporter related-gene expression were investigated via experiments. The results showed that under 100 μmol/L copper stress, the root length and fresh weight of tobacco seedlings increased comparing with those of untreated seedlings. Under 0.5 mmol/L copper stress, 0.2 and 0.5 mmol/L BABA treatments decreased the contents of malonaldehyde (MDA) and hydrogen peroxide (H2O2), improved the activities of antioxidative enzymes (SOD, POD and APX), meanwhile the expression levels of NtNRAMP, NtYSL and NtPDR genes, which regulated the transportation of divalent metal ions in tobacco seedlings, significantly increased. It indicated that BABA alleviated the damage of tobacco caused by copper stress through regulating the antioxidant system and the expression levels of genes related to the transportation of metal ions.
