CAJ | 학술논문

为了明确烟草β-胡萝卜素羟化酶（β-carotene hydroxylase，β-OHase）基因的转录表达模式和功能，进而调控烟草类胡萝卜素的代谢,通过Real-time PCR分析了普通烟草（Nicotiana tabacum）品种红花大金元盛花期的β-OHase基因在不同器官中的表达量。利用烟草脆裂病毒诱导的基因沉默（VIGS）体系抑制本氏烟草（Nicotiana benthamiana）中β-OHase基因的表达，在该基因成功沉默后，通过Real-time PCR检测其下游基因表达量，同时检测烟草中β-胡萝卜素、紫黄质和新黄质含量(质量分数)。结果显示，β-OHase基因在叶片中的表达量明显高于其他器官（P＜0.05）。与对照相比，在该基因沉默后，下游的紫黄质脱环氧化酶基因（VDE）和玉米黄质环氧化酶基因（ZE）的表达量明显降低，同时β-胡萝卜素含量显著升高，而紫黄质和新黄质含量则显著降低（P＜0.05）。
위료명학연초β-호라복소간화매（β-carotene hydroxylase，β-OHase）기인적전록표체모식화공능，진이조공연초류호라복소적대사,통과Real-time PCR분석료보통연초（Nicotiana tabacum）품충홍화대금원성화기적β-OHase기인재불동기관중적표체량。이용연초취렬병독유도적기인침묵（VIGS）체계억제본씨연초（Nicotiana benthamiana）중β-OHase기인적표체，재해기인성공침묵후，통과Real-time PCR검측기하유기인표체량，동시검측연초중β-호라복소、자황질화신황질함량(질량분수)。결과현시，β-OHase기인재협편중적표체량명현고우기타기관（P＜0.05）。여대조상비，재해기인침묵후，하유적자황질탈배양화매기인（VDE）화옥미황질배양화매기인（ZE）적표체량명현강저，동시β-호라복소함량현저승고，이자황질화신황질함량칙현저강저（P＜0.05）。
In order to clarify the transcription expression pattern and functions of β-carotene hydroxylase gene (β-OHase) in Nicotiana tabacum and further to regulate the metabolism of carotenoids in tobacco, the expression level of β-OHase in different tissues and organs of cv. Honghuadajinyuan (Nicotiana tabacum) at full flowering stage was studied by Real-time PCR. On account of the depression of β-OHase expression in Nicotiana benthamiana via the gene silencing system induced by tobacco rattle virus, the expression levels of downstream genes (such as VDE & ZE) were detected by Real-time PCR and the contents of carotenoids (β-carotene, violaxanthin and neoxanthin) in tobacco were determined after β-OHase silencing. The results showed that the expression level of β-OHase was significantly higher (P<0.05) in leaves than in the other organs. Comparing with the control, the expression levels of VDE & ZE and the contents of violaxanthin and neoxanthin significantly decreased, while β-carotene content obviously increased (P<0.05) after β-OHase silencing.