CAJ | 학술논문

酚氧化酶是一种含铜的金属酶，广泛分布于微生物、动植物及人体中。该酶在无脊椎动物先天免疫防御中起着重要的作用。用分子克隆技术得到了虾夷扇贝酚氧化酶cDNA基因全序列，并利用Real‐TimePCR方法分析了酚氧化酶基因在虾夷扇贝不同组织中表达概况以及鳗弧菌注射后不同时段的相对表达量。试验得到的酚氧化酶基因全长1850bp，其包括长为1470bp的开放阅读框，编码489个氨基酸，5’UTR和3’UTR分别为37bp和343bp。实时定量分析研究显示，酚氧化酶基因在肝胰腺、外套膜、肾、闭壳肌、鳃和血中均有表达，其中外套膜表达最高。鳗弧菌注射后6h，酚氧化酶基因表达量显著低于对照组，而注射后12～36h，酚氧化酶基因表达量显著高于对照组，表明该基因可能在免疫过程中发挥重要作用。
분양화매시일충함동적금속매，엄범분포우미생물、동식물급인체중。해매재무척추동물선천면역방어중기착중요적작용。용분자극륭기술득도료하이선패분양화매cDNA기인전서렬，병이용Real‐TimePCR방법분석료분양화매기인재하이선패불동조직중표체개황이급만호균주사후불동시단적상대표체량。시험득도적분양화매기인전장1850bp，기포괄장위1470bp적개방열독광，편마489개안기산，5’UTR화3’UTR분별위37bp화343bp。실시정량분석연구현시，분양화매기인재간이선、외투막、신、폐각기、새화혈중균유표체，기중외투막표체최고。만호균주사후6h，분양화매기인표체량현저저우대조조，이주사후12～36h，분양화매기인표체량현저고우대조조，표명해기인가능재면역과정중발휘중요작용。
In this study ,tyrosinase (MyTYR) gene was sequenced from Japanese scallop Patinopecten yessoensis by molecular cloning technique ,and MyTYR expression was studied in different tissues in the Japanese scallop challenged with bacterial pathogen (Vibrio anguillarum) at various periods of injection by quantitative real time PCR (qRT‐PCR) .The full‐length 1850 bp MyTYR mRNA contained a 37 bp 5’ untranslated region ,a 1470 open reading frame encoding 489 amino acids and a 343 bp 3’ UTR .The qRT‐PCR showed that MyTYR gene was expressed in the hepatopancreas ,mantle ,kidney ,adductor ,gill and hemocytes ,the maximum in the mantle .There was significantly lower quantity of TYR gene expression 6 hours after the bacterial pathogen injection than that in the control group .From twelve to thirty‐six hours after injection ,however ,the expression level of TYR gene was significantly higher than that in the control group ,indicating that the gene may play an important role in immune process .