现代医药卫生
現代醫藥衛生
현대의약위생
MODERN MEDICINE HEALTH
2015年
8期
1131-1133,1136
,共4页
晶体%紫外线%白内障%超氧化物歧化酶%谷胱甘肽过氧化酶%过氧化氢酶%大鼠,Sprague-Dawley
晶體%紫外線%白內障%超氧化物歧化酶%穀胱甘肽過氧化酶%過氧化氫酶%大鼠,Sprague-Dawley
정체%자외선%백내장%초양화물기화매%곡광감태과양화매%과양화경매%대서,Sprague-Dawley
Lens,crystalline%Ultraviolet rays%Cataract%Superoxide dismutase%Glutathione peroxidase%Catalase%Rats,sprague-dawley
目的：以紫外线照射SD大鼠为研究模型，观察紫外线对晶状体中抗氧化酶[（超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）]活性的影响。方法将30只6周龄SD大鼠随机分为五组，每组6只，对照组不做处理，其余四组实验组腹腔注射水合氯醛（10%，0.35 mL/100 g）麻醉大鼠，紫外灯正对大鼠眼球照射15 min，照射强度1.0×103μW/cm2。分别于紫外线照射后1、3、5、7 d颈椎脱臼处死SD大鼠，立即取出晶状体，观察晶状体是否浑浊，并拍照记录；比色法检测晶状体中SOD、GSH-Px、CAT 活性及超氧阴离子（O2-）、过氧化氢（H2O2）、丙二醛（MDA）含量。结果紫外线照射后晶状体的浑浊程度随时间延长而逐渐加重；晶状体中SOD活性较对照组显著降低，而O2-含量显著升高，GSH-Px活性显著降低，差异均有统计学意义（P＜0.01）；紫外线照射后3、5、7 d晶状体中CAT活性较对照组显著降低，而H2O2含量较对照组显著升高，差异均有统计学意义（P＜0.01）；紫外线照射后晶状体中MDA含量较对照组升高，差异有统计学意义（P＜0.01）。结论紫外线诱导白内障的发生与晶状体中抗氧化酶（SOD、GSH-Px、CAT）活性降低有关。
目的：以紫外線照射SD大鼠為研究模型，觀察紫外線對晶狀體中抗氧化酶[（超氧化物歧化酶（SOD）、穀胱甘肽過氧化物酶（GSH-Px）、過氧化氫酶（CAT）]活性的影響。方法將30隻6週齡SD大鼠隨機分為五組，每組6隻，對照組不做處理，其餘四組實驗組腹腔註射水閤氯醛（10%，0.35 mL/100 g）痳醉大鼠，紫外燈正對大鼠眼毬照射15 min，照射彊度1.0×103μW/cm2。分彆于紫外線照射後1、3、5、7 d頸椎脫臼處死SD大鼠，立即取齣晶狀體，觀察晶狀體是否渾濁，併拍照記錄；比色法檢測晶狀體中SOD、GSH-Px、CAT 活性及超氧陰離子（O2-）、過氧化氫（H2O2）、丙二醛（MDA）含量。結果紫外線照射後晶狀體的渾濁程度隨時間延長而逐漸加重；晶狀體中SOD活性較對照組顯著降低，而O2-含量顯著升高，GSH-Px活性顯著降低，差異均有統計學意義（P＜0.01）；紫外線照射後3、5、7 d晶狀體中CAT活性較對照組顯著降低，而H2O2含量較對照組顯著升高，差異均有統計學意義（P＜0.01）；紫外線照射後晶狀體中MDA含量較對照組升高，差異有統計學意義（P＜0.01）。結論紫外線誘導白內障的髮生與晶狀體中抗氧化酶（SOD、GSH-Px、CAT）活性降低有關。
목적：이자외선조사SD대서위연구모형，관찰자외선대정상체중항양화매[（초양화물기화매（SOD）、곡광감태과양화물매（GSH-Px）、과양화경매（CAT）]활성적영향。방법장30지6주령SD대서수궤분위오조，매조6지，대조조불주처리，기여사조실험조복강주사수합록철（10%，0.35 mL/100 g）마취대서，자외등정대대서안구조사15 min，조사강도1.0×103μW/cm2。분별우자외선조사후1、3、5、7 d경추탈구처사SD대서，립즉취출정상체，관찰정상체시부혼탁，병박조기록；비색법검측정상체중SOD、GSH-Px、CAT 활성급초양음리자（O2-）、과양화경（H2O2）、병이철（MDA）함량。결과자외선조사후정상체적혼탁정도수시간연장이축점가중；정상체중SOD활성교대조조현저강저，이O2-함량현저승고，GSH-Px활성현저강저，차이균유통계학의의（P＜0.01）；자외선조사후3、5、7 d정상체중CAT활성교대조조현저강저，이H2O2함량교대조조현저승고，차이균유통계학의의（P＜0.01）；자외선조사후정상체중MDA함량교대조조승고，차이유통계학의의（P＜0.01）。결론자외선유도백내장적발생여정상체중항양화매（SOD、GSH-Px、CAT）활성강저유관。
Objective To observe the effect of ultraviolet radiation on antioxidant enzymes activities (SOD,GSH-Px,CAT) with the research model of SD rats under the ultraviolet irradiation. Methods A total of 30 SD rats aged 6 months were randomly divided into 5 groups,6 rats in each group,including one as the control group without any disposal and four as the experimental groups,who were injected 10%chloral hydrate(0.35 mL/100 g) abdominally for anesthesia. Using UV lamps radiated SD rats′eyes for 15 minutes after pupillary dilation with the exposure intensity being 1.0 ×103μW/cm2,the exposed animals were put to death by cervical dislocation on the first,third,fifth and seventh day respectively after exposure and then the lenses from the rats were extracted as soon as possible. It was observed whether the lenses were muddy or not ,taking pictures to be recorded. The colorimetry was used to determine the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),catalase(CAT) and the content of O2-,H2O2,malondialdehyde(MDA). Results After exposure under ultraviolet radiation,the longer the expo-sure period was,the heavier the muddy degree become. It was decreased in SOD activities of the lenses than that of the control group while increased in O2-content significantly,and declined distinctively in GSH-Px activities. The difference had statistical significance(P<0.01). On the third,fifth and seventh day,the CAT activities in the lenses was lower compared with the control group while the H2O2 content was significantly increased compared with the control group. The difference had statistical signifi-cance(P<0.01). The MDA content in the lenses after ultraviolet exposure,The difference had statistical significance(P<0.01). Conclusion The occurrence of UVB-induced cataract plays an important role in SOD,GSH-Px,CAT in the lenses.
