烟草科技
煙草科技
연초과기
TOBACCO SCIENCE & TECHNOLOGY
2015年
4期
49-55
,共7页
卷烟%滤嘴%截留%主流烟气%酚类化合物%高效液相色谱法
捲煙%濾嘴%截留%主流煙氣%酚類化閤物%高效液相色譜法
권연%려취%절류%주류연기%분류화합물%고효액상색보법
Cigarette%Filter%Retention%Mainstream cigarette smoke%Phenolic compound%High performance liquid chromatography
为准确测定卷烟滤嘴截留主流烟气中的主要酚类化合物，通过考察滤嘴切割方式、萃取液、萃取方式、萃取时间等前处理条件，建立了一种快速、准确测定卷烟滤嘴中对苯二酚、间苯二酚、邻苯二酚、苯酚、对甲酚、间甲酚和邻甲酚7种酚类化合物截留量的高效液相色谱(HPLC)方法，并采用该方法在6家实验室对7种不同滤嘴卷烟样品进行了比对实验。结果表明：①将卷烟抽吸后的滤嘴从烟支端向近嘴端纵向划开后，用50 mL萃取液（1%乙酸+79%甲醇+20%水，体积分数）在室温下超声萃取30 min时，可将卷烟滤嘴中截留的7种主要酚类化合物萃取完全。②7种酚类化合物在0.04~4.00μg/mL浓度范围内线性关系良好（R2均大于0.999），回收率为93.35%~104.50%，相对标准偏差（RSD）小于7.3%。③6家实验室对7种卷烟样品滤嘴截留的7种酚类化合物检测结果的RSD均小于15%，其中5种酚类化合物在10%以下。该方法适用于卷烟滤嘴截留主流烟气中7种酚类化合物的测定。
為準確測定捲煙濾嘴截留主流煙氣中的主要酚類化閤物，通過攷察濾嘴切割方式、萃取液、萃取方式、萃取時間等前處理條件，建立瞭一種快速、準確測定捲煙濾嘴中對苯二酚、間苯二酚、鄰苯二酚、苯酚、對甲酚、間甲酚和鄰甲酚7種酚類化閤物截留量的高效液相色譜(HPLC)方法，併採用該方法在6傢實驗室對7種不同濾嘴捲煙樣品進行瞭比對實驗。結果錶明：①將捲煙抽吸後的濾嘴從煙支耑嚮近嘴耑縱嚮劃開後，用50 mL萃取液（1%乙痠+79%甲醇+20%水，體積分數）在室溫下超聲萃取30 min時，可將捲煙濾嘴中截留的7種主要酚類化閤物萃取完全。②7種酚類化閤物在0.04~4.00μg/mL濃度範圍內線性關繫良好（R2均大于0.999），迴收率為93.35%~104.50%，相對標準偏差（RSD）小于7.3%。③6傢實驗室對7種捲煙樣品濾嘴截留的7種酚類化閤物檢測結果的RSD均小于15%，其中5種酚類化閤物在10%以下。該方法適用于捲煙濾嘴截留主流煙氣中7種酚類化閤物的測定。
위준학측정권연려취절류주류연기중적주요분류화합물，통과고찰려취절할방식、췌취액、췌취방식、췌취시간등전처리조건，건립료일충쾌속、준학측정권연려취중대분이분、간분이분、린분이분、분분、대갑분、간갑분화린갑분7충분류화합물절류량적고효액상색보(HPLC)방법，병채용해방법재6가실험실대7충불동려취권연양품진행료비대실험。결과표명：①장권연추흡후적려취종연지단향근취단종향화개후，용50 mL췌취액（1%을산+79%갑순+20%수，체적분수）재실온하초성췌취30 min시，가장권연려취중절류적7충주요분류화합물췌취완전。②7충분류화합물재0.04~4.00μg/mL농도범위내선성관계량호（R2균대우0.999），회수솔위93.35%~104.50%，상대표준편차（RSD）소우7.3%。③6가실험실대7충권연양품려취절류적7충분류화합물검측결과적RSD균소우15%，기중5충분류화합물재10%이하。해방법괄용우권연려취절류주류연기중7충분류화합물적측정。
In order to accurately determine the major phenolic compounds (hydroquinol, resorcinol, catechol, phenol, p-cresol, m-cresol, o-cresol) retained by cigarette filters, a rapid and accurate high performance liquid chromatography (HPLC) method was developed by investigating sample pretreatment conditions, such as filter-slitting means, extraction solvent, extraction method, and extraction time. A comparative experiment on seven filter cigarette samples was performed with the developed method among six laboratories. The results showed that: 1) By slitting the filter longitudinally from the tobacco end to the mouth end, then subjecting it to ultrasonic extraction with 50 mL solution (1% acetic acid, 79% methanol and 20% water, V/V) for 30 minutes at room temperature, the said phenolic compounds retained in the filter could be completely extracted. 2) Within the concentration of 0.04-4.00 μg/mL, the said phenolic compounds exhibited good linear relations with R2 > 0.999, the recoveries ranged from 93.35% to 104.50% with relative standard deviations (RSDs) < 7.3%. 3) The RSD of the six laboratories in resorcinol and phenol determination was less than 15%, in the other five phenolic compounds determination was less than 10%. This method is suitable for determining the said phenolic compounds in mainstream cigarette smoke retained by cigarette filter.
