CAJ | 학술논문

目的：观察血管生成抑制素治疗血管瘤的效果。方法发酵毕赤酵母工程菌组成型表达重组血管生成抑制素，用SP-Sepharose Fast Flow (阳离子交换剂)柱进行蛋白纯化；通过抑制鸡胚绒毛尿囊膜(CAM)血管生成试验和诱发血管内皮细胞凋亡试验，分析重组血管生成抑制素的生物学活性，在血管瘤模型(公鸡肉垂)中注射血管生成抑制素，分析血管生成抑制素对血管瘤的药效。结果血管生成抑制素在毕赤酵母中高效表达，经过纯化，收获达98%的重组血管生成抑制素；重组血管生成抑制素具有抑制CAM血管生成和诱发血管内皮细胞凋亡的活性；重组血管生成抑制素与血管瘤模型作用14 d后，瘤体血管生长抑制率为51%(P<0.01)，血管瘤增殖抑制率为39%(P<0.05)。结论成功制备重组血管生成抑制素药物，血管生成抑制素对于血管瘤模型具有显著的药效。
목적：관찰혈관생성억제소치료혈관류적효과。방법발효필적효모공정균조성형표체중조혈관생성억제소，용SP-Sepharose Fast Flow (양리자교환제)주진행단백순화；통과억제계배융모뇨낭막(CAM)혈관생성시험화유발혈관내피세포조망시험，분석중조혈관생성억제소적생물학활성，재혈관류모형(공계육수)중주사혈관생성억제소，분석혈관생성억제소대혈관류적약효。결과혈관생성억제소재필적효모중고효표체，경과순화，수획체98%적중조혈관생성억제소；중조혈관생성억제소구유억제CAM혈관생성화유발혈관내피세포조망적활성；중조혈관생성억제소여혈관류모형작용14 d후，류체혈관생장억제솔위51%(P<0.01)，혈관류증식억제솔위39%(P<0.05)。결론성공제비중조혈관생성억제소약물，혈관생성억제소대우혈관류모형구유현저적약효。
Objective To observe the effect of using angiostatin to treat angeioma. Methods Recombinant angiostatin was constitutively expressed in Pichia pastoris by fermentation and then purified by SP-Sepharose Fast Flow (cation exchanger). Biological activity of the recombinant angiostatin was analyzed by the tests of suppression angiogenesis of chorioallantoic membrane of chick embry (CAM) and inducing apoptosis of vascular endothelial cells. The drug effect for angeioma was analyzed after injecting recombinant angiostatin into the angeioma model (cock wattle). Results Angiostatin showed high-efficiency expression in Pichia pastoris and 98%of recombinant an-giostatin had been obtained after the purification. The recombinant angiostatin had activity on suppressing of angiogen-esis on CAM and inducing of vascular endothelial cells apoptosis. It's 51%(P<0.01) of the inhibition rate of vascular growth in the angeioma model and 39%(P<0.05) of the inhibition rate of proliferation of the hemangioma model after 14 days of maintenance therapy. Conclusion Drug of recombinant angiostatin had been successfully prepared. Angi-ostatin possesses prominent effect on the treatment of angeioma.