CAJ | 학술논문

目的：利用复合松质骨基质(Cancellous bone matrix ，CBM)的中空多孔钛假体(Hollow porous titanium prostheses, HPTP)观察兔体内假体周围及内部骨生成情况，探讨人工假体与骨质整合的改进方法。方法设计中空多孔(孔径2 mm)和无孔两种假体，表面行羟基磷灰石(Hydroxyapatite ，HA)喷涂。实验组分为无孔假体组(A组)、HPTP组(B组)和HPTP＋CBM组(C组)，每组16例。将假体分别植入48只4~6个月龄新西兰大白兔右侧股骨外侧髁，饲养至第3、8、12周时取材，以X线、显微镜、电镜及形态计量软件观察复合假体表面及内部骨生成情况。采用SPSS13.0进行t检验分析。结果三组植入物外表面HA涂层的组织生长情况类似；A组无孔，未见骨长入，B组和C组中骨组织最终可长入假体2 mm大的圆孔；各时间点C组中空腔内骨生长率均明显高于B组(P<0.01)。结论骨质可经HPTP假体孔洞长入腔内，达到交锁固定，较无孔假体更稳定；中空腔复合CBM后可明显加快骨长入并诱导骨生成，使假体与骨质更好整合。
목적：이용복합송질골기질(Cancellous bone matrix ，CBM)적중공다공태가체(Hollow porous titanium prostheses, HPTP)관찰토체내가체주위급내부골생성정황，탐토인공가체여골질정합적개진방법。방법설계중공다공(공경2 mm)화무공량충가체，표면행간기린회석(Hydroxyapatite ，HA)분도。실험조분위무공가체조(A조)、HPTP조(B조)화HPTP＋CBM조(C조)，매조16례。장가체분별식입48지4~6개월령신서란대백토우측고골외측과，사양지제3、8、12주시취재，이X선、현미경、전경급형태계량연건관찰복합가체표면급내부골생성정황。채용SPSS13.0진행t검험분석。결과삼조식입물외표면HA도층적조직생장정황유사；A조무공，미견골장입，B조화C조중골조직최종가장입가체2 mm대적원공；각시간점C조중공강내골생장솔균명현고우B조(P<0.01)。결론골질가경HPTP가체공동장입강내，체도교쇄고정，교무공가체경은정；중공강복합CBM후가명현가쾌골장입병유도골생성，사가체여골질경호정합。
Objective To observe the possibility of bone formation in vivo of rabbits with hollow porous tita-nium prostheses (HPTP) loading cancellous bone matrix (CBM), so as to improve the osteointegration between pros-theses and bone. Methods HPTP (2 mm in diameter) and non-porous prostheses coated with HA were designed. Ex-perimental samples were divided into non-porous prosthesis group (group A), HPTP group (group B) and HPTP plus CBM group (group C), with 16 samples in each group. All the implants were implanted in the right lateral femoral con-dyles of 48 New Zealand rabbits of 4~6 months old, which were fed to 3, 8, 12 weeks later and sacrificed. SPSS13.0 software was used for t-test analysis. Results The tissues around HA-coated implants in the three groups were nearly the same at different time points. No bone grew into Group A because of non-porous design, and bone could grow into the 2 mm round holes both in group B and C ultimately. Bone formation rates in group C were significantly higher than that in group B at different time points (P<0.01). Conclusion Bone could grow into the cavity of HPTP pros-theses via the round holes to achieve locking fixation effect, which are more stable than non-porous prostheses. CBM could speed up bone ingrowth significantly and induce bone formation to achieve better integration of pros-theses and bone.