西南大学学报（自然科学版）
西南大學學報（自然科學版）
서남대학학보（자연과학판）
JOURNAL OF SOUTHWEST AGRICULTURAL UNIVERSITY(NATURAL SCIENCE)
2015年
4期
158-163
,共6页
微流控芯片%介电电泳%叉指式阵列微电极%电场模拟%细胞富集
微流控芯片%介電電泳%扠指式陣列微電極%電場模擬%細胞富集
미류공심편%개전전영%차지식진렬미전겁%전장모의%세포부집
microfluidic chip%dielectrophoresis%interdigitated array microelectrode%electric field simula-tion%cell enrichment
为了研制高富集效率的介电电泳细胞分析芯片，首先从介电电泳力出发，推导了悬浮细胞所受的介电电泳力公式．通过对比常规微电极的电场强度，选择叉指式阵列微电极构建介电电泳芯片模型，对不同结构参数下微通道中的电场分布进行模拟，由此对芯片结构参数进行优化设计．为了对模拟结果进行验证，采用微加工技术制作了介电电泳细胞分析芯片．以HepG2肝癌细胞为待测样品，当芯片所施加正弦交流电压为5 V ，频率为4 M Hz时，获得了88.89％的富集效率．
為瞭研製高富集效率的介電電泳細胞分析芯片，首先從介電電泳力齣髮，推導瞭懸浮細胞所受的介電電泳力公式．通過對比常規微電極的電場彊度，選擇扠指式陣列微電極構建介電電泳芯片模型，對不同結構參數下微通道中的電場分佈進行模擬，由此對芯片結構參數進行優化設計．為瞭對模擬結果進行驗證，採用微加工技術製作瞭介電電泳細胞分析芯片．以HepG2肝癌細胞為待測樣品，噹芯片所施加正絃交流電壓為5 V ，頻率為4 M Hz時，穫得瞭88.89％的富集效率．
위료연제고부집효솔적개전전영세포분석심편，수선종개전전영력출발，추도료현부세포소수적개전전영력공식．통과대비상규미전겁적전장강도，선택차지식진렬미전겁구건개전전영심편모형，대불동결구삼수하미통도중적전장분포진행모의，유차대심편결구삼수진행우화설계．위료대모의결과진행험증，채용미가공기술제작료개전전영세포분석심편．이HepG2간암세포위대측양품，당심편소시가정현교류전압위5 V ，빈솔위4 M Hz시，획득료88.89％적부집효솔．
In order to fabricate dielectrophoresis (DEP) chips with higher enrichment efficiency ,a formula of the dielectrophoresis force applied in suspended cells was theoretically deduced .After comparing the e‐lectric field contribution for conventional micro electrodes ,the interdigitated array microelectrodes were chosen to model the dielectrophoresis chip .The parameters for the dielectrophoresis chip were designed and optimized by COMSOL Multiphysics software .In order to validate the simulation results ,the micro processing technology was used to fabricate the dielectrophoresis cell analysis chip .HepG2 hepatoma carci‐noma cells were used as the testing sample .When the sinusoidal alternating voltage with a peak‐to‐peak amplitude of 5V and a frequency of 4M Hz was applied on the dielectrophoresis chip ,the enrichment effi‐ciency for the HepG2 hepatoma carcinoma cells was as high as 88.89% .
