西南大学学报（自然科学版）
西南大學學報（自然科學版）
서남대학학보（자연과학판）
JOURNAL OF SOUTHWEST AGRICULTURAL UNIVERSITY(NATURAL SCIENCE)
2015年
4期
170-176
,共7页
霉酚酸%超临界CO2 萃取%解析压力%解析温度%响应面分析
黴酚痠%超臨界CO2 萃取%解析壓力%解析溫度%響應麵分析
매분산%초림계CO2 췌취%해석압력%해석온도%향응면분석
mycophenolic acid%supercritical CO2 extraction%separation pressure%separation temperature%response surface analysis
采用超临界CO2萃取霉酚酸，通过调节分离釜Ⅰ的压力和温度，增加萃取物的解析选择性，提高分离釜Ⅰ中解析物的霉酚酸纯度．采用2因素5水平的星点设计试验，考察了分离釜Ⅰ的压力和温度对萃取物的霉酚酸含量（CMPA ）和霉酚酸萃取回收率（RR）的影响；响应面分析显示，RR与分离釜Ⅰ的压力和温度符合二次模型，压力的一次项和二次项均对 RR有极显著影响，温度的一次项也对 RR有较显著影响；而 CMPA与分离釜Ⅰ的压力和温度符合线性方程，压力对 CMPA有极显著影响．根据拟合方程预测分离釜Ⅰ的最佳压力和温度，综合考虑 RR和CMPA ，确定为14.69 M Pa和69.99℃．验证实验中设定分离釜Ⅰ的压力为14.5 M Pa、温度为70℃， RR 为（91.57±1.31）％，CMPA为（77.50±1.03）％，与预测值90.96％和76.49％接近．
採用超臨界CO2萃取黴酚痠，通過調節分離釜Ⅰ的壓力和溫度，增加萃取物的解析選擇性，提高分離釜Ⅰ中解析物的黴酚痠純度．採用2因素5水平的星點設計試驗，攷察瞭分離釜Ⅰ的壓力和溫度對萃取物的黴酚痠含量（CMPA ）和黴酚痠萃取迴收率（RR）的影響；響應麵分析顯示，RR與分離釜Ⅰ的壓力和溫度符閤二次模型，壓力的一次項和二次項均對 RR有極顯著影響，溫度的一次項也對 RR有較顯著影響；而 CMPA與分離釜Ⅰ的壓力和溫度符閤線性方程，壓力對 CMPA有極顯著影響．根據擬閤方程預測分離釜Ⅰ的最佳壓力和溫度，綜閤攷慮 RR和CMPA ，確定為14.69 M Pa和69.99℃．驗證實驗中設定分離釜Ⅰ的壓力為14.5 M Pa、溫度為70℃， RR 為（91.57±1.31）％，CMPA為（77.50±1.03）％，與預測值90.96％和76.49％接近．
채용초림계CO2췌취매분산，통과조절분리부Ⅰ적압력화온도，증가췌취물적해석선택성，제고분리부Ⅰ중해석물적매분산순도．채용2인소5수평적성점설계시험，고찰료분리부Ⅰ적압력화온도대췌취물적매분산함량（CMPA ）화매분산췌취회수솔（RR）적영향；향응면분석현시，RR여분리부Ⅰ적압력화온도부합이차모형，압력적일차항화이차항균대 RR유겁현저영향，온도적일차항야대 RR유교현저영향；이 CMPA여분리부Ⅰ적압력화온도부합선성방정，압력대 CMPA유겁현저영향．근거의합방정예측분리부Ⅰ적최가압력화온도，종합고필 RR화CMPA ，학정위14.69 M Pa화69.99℃．험증실험중설정분리부Ⅰ적압력위14.5 M Pa、온도위70℃， RR 위（91.57±1.31）％，CMPA위（77.50±1.03）％，여예측치90.96％화76.49％접근．
In supercritical CO2 extraction of mycophenolic acid (MPA) ,to improve the separation selectivi‐ty of MPA in separation tank I ,central composite design was used to study the effects of pressure and temperature in separation tank I on the recovery rate (RR) and the content of M PA (CMPA ) collected in separation tank I .A quadratic formula was obtained by response surface analysis to describe the relation‐ships between RR and pressure and temperature in separation tank I ,and a linear formula to describe the relationships between CMPA and the two independent variables .The linear term and quadratic term of pres‐sure had highly significant effects on RR ,and the linear term of temperature influenced RR significantly , too ;whereas only the linear term of pressure had highly significant effect on CMPA .Taking RR and CMPA in‐to account simultaneously ,the optimum pressure and temperature in separation tank I were determined , according to the fitting polynomial models ,to be 14.69 MPa and 69.99 ℃ ,respectively .Three verification tests were carried out under the condition of 14.5 MPa and 70 ℃ in separation tank I ,and the RR and CMPA were (91.57 ± 1.31)% and (77.50 ± 1.03)% ,respectively ,which well matched the predicted values , 90.96% and 76.49% ,respectively .
