CAJ | 학술논문

采用无水乙醇对无患子果皮中的皂素进行提取分离。提取工艺研究表明,当固液比为1：6,在50℃,150 r/ min 摇床中提取6 h 后,无患子皂素的得率为37.5%。通过向提取液中分别加入丙酮和无水乙醚可将皂素析出分离,皂素得率分别为17.9%和32.0%。丙酮析出分离出的皂素得率较低,但疏松洁白,颗粒较细,纯度为90.5%。无水乙醚析出分离的皂素颜色微黄,颗粒较大,纯度为84.7%,得率高达85.3%,是理想的无患子皂素提纯溶剂。
채용무수을순대무환자과피중적조소진행제취분리。제취공예연구표명,당고액비위1：6,재50℃,150 r/ min 요상중제취6 h 후,무환자조소적득솔위37.5%。통과향제취액중분별가입병동화무수을미가장조소석출분리,조소득솔분별위17.9%화32.0%。병동석출분리출적조소득솔교저,단소송길백,과립교세,순도위90.5%。무수을미석출분리적조소안색미황,과립교대,순도위84.7%,득솔고체85.3%,시이상적무환자조소제순용제。
The saponin was extracted from Sapindus mukurossi Gaertn. using anhydrous ethanol as ex-traction solvent. The effects of temperature, solid-liquid ratio and time on extraction efficiency were in-vestigated. The results showed that the optimal conditions for the extraction of saonin were temperature of 50 ℃, solid-liquid ratio of 1 ∶ 6 and time of 6 h. At the optimal conditions, the yield of Sapindus muku-rossi saponin reached 37. 5% . The isolation of saponin from the ethanol extraction was carried out by the addition of acetone or anhydrous ether, yielding 17. 9% and 32. 0% of sapindosides (on the basis of the weight of pericarps), respectively. The saponin precipitated by acetone with a purity of 90. 5% were por-ous and fine-grained with white color, while the saponin obtained from anhydrous ether precipitation with a purity of 84. 7% were yellowish with lager particle size. The precipitation rate of saponin from the etha-nolic extract by anhydrous ether was 85. 3% , which suggested the potential of anhydrous ether for the iso-lation of saponin.