CAJ | 학술논문

以思茅松无性系种子园收集的来自7个居群的85个思茅松用材林优良无性系为试验材料，使用2×Taq PCR MasterMix并利用Touchdown PCR构建思茅松RAPD－PCR反应体系。从120条引物中共筛选出适用于思茅松遗传多样性分析的20条引物进行PCR扩增，来自不同种源地7个居群的多态位点百分率PPB平均值59．48％；平均每个位点的有效等位基因数Ne平均值1．3503； Nei’s基因多样指数H平均值0．2063； Shannon’s多样性信息指数I平均值0．3100。通过对7个居群的遗传距离进行分析，并构建UPGMA聚类树状图，从聚类结果看，7个居群可以划分为遗传差异较明显的3大类群。
이사모송무성계충자완수집적래자7개거군적85개사모송용재림우량무성계위시험재료，사용2×Taq PCR MasterMix병이용Touchdown PCR구건사모송RAPD－PCR반응체계。종120조인물중공사선출괄용우사모송유전다양성분석적20조인물진행PCR확증，래자불동충원지7개거군적다태위점백분솔PPB평균치59．48％；평균매개위점적유효등위기인수Ne평균치1．3503； Nei’s기인다양지수H평균치0．2063； Shannon’s다양성신식지수I평균치0．3100。통과대7개거군적유전거리진행분석，병구건UPGMA취류수상도，종취류결과간，7개거군가이화분위유전차이교명현적3대류군。
As the study materials , 85 Pinus kesiya var.langbianensis clones of 7 populations were collected from the clonal seed orchard .By adopting the method of 2 ×Taq PCR MasterMix amplification and Touchdown PCR , RAPD reaction systems was tested .Twenty primers with high stability and polymorphism were screened out from 120 RAPD primers which could be used for all samples of RAPD-PCR amplification .The results showed that , for the 7 population level , the percentage of polymorphic bands average was 59.48%, the number of effective alleles per band Ne average was 1.350 3 , the Nei ’ s gene diversity index H average was 0.206 3 , the Shannon ’ s diversity index I average was 0.310 0.The genetic distance was analyzed and UPGMA were clustered at the 7 population, and the study results revealed these 7 population could be divided into 3 groups.