CAJ | 학술논문

目的：建立免疫抑制小鼠侵袭性肺曲霉菌病（IPA）动物模型，为阐明IPA的发病机制和临床治疗提供基础研究。方法用环磷酰胺造成小鼠免疫抑制，双侧鼻孔滴入烟曲霉菌孢子，分别于24 h、48 h、72 h、96 h不同时间点处死小鼠，通过肺组织病理、肺组织真菌培养确定侵袭性肺曲霉菌病模型是否构建成功。结果 IPA 模型组的组织培养可见烟曲霉菌，病理切片模型组小鼠72 h时可见较严重的出血和充血；96 h时肺内有菌丝，肺泡间隔增宽，组织坏死。正常状态感染烟曲霉小鼠72 h可见充血，肺泡弹性纤维被破坏；96 h仅表现为炎症和出血，未见菌丝。结论成功建立了小鼠IPA动物模型，为研究侵袭性曲霉菌病的发病机制、早期诊断和防治奠定了基础。
목적：건립면역억제소서침습성폐곡매균병（IPA）동물모형，위천명IPA적발병궤제화림상치료제공기출연구。방법용배린선알조성소서면역억제，쌍측비공적입연곡매균포자，분별우24 h、48 h、72 h、96 h불동시간점처사소서，통과폐조직병리、폐조직진균배양학정침습성폐곡매균병모형시부구건성공。결과 IPA 모형조적조직배양가견연곡매균，병리절편모형조소서72 h시가견교엄중적출혈화충혈；96 h시폐내유균사，폐포간격증관，조직배사。정상상태감염연곡매소서72 h가견충혈，폐포탄성섬유피파배；96 h부표현위염증화출혈，미견균사。결론성공건립료소서IPA동물모형，위연구침습성곡매균병적발병궤제、조기진단화방치전정료기출。
Objective To establish an experimental animal model of invasive pulmonary aspergillosis in immunosuppressed mice, and to elucidate the pathogenesis and clinical treatment of IPA. Methods Immunosuppressed mice were induced by cyclophosphamide intrapcritoncal injections and the Aspergillus fumigatus conidia were dropped into the nares of each mouse to cause invasive pulmonary aspergillosis. All mice were killed at different time points, 24 h, 48 h, 72 h and 96 h, respectively, Through the lung tissue histopathologically analysis and fungal culture to evaluate aspergillosis model. Results The IPA model group showed visible Aspergillus fumigatus, and the IPA mice infected after 72 h showed severe bleeding and congestive, and lung tissue granuloma formation;the 96 h group, showed hyphae, widened alveolar septum, tracheal epithelium cell loss, tissue necrosis. Normal mice infected by Aspergillus fumigatus after 72 h showed visible congestion and destroyed alveolar elastic fibers, however, the mice infected after 96 h just showed inflammation and bleeding, no hyphae. Conclusion Establishing the IPA animal model of the mice, and may provide the foundation for the study of the aspergillosis pathogenesis, early diagnosis and prevention of the aspergillosis.