CAJ | 학술논문

目的建立兔肝癌模型，观察实验动物对叶酸（ FA ）偶联二氧化硅包覆的金纳米棒（GNRs@SiO2-FA）的摄取状况，检测GNRs@SiO2-FA对肝癌细胞的靶向性。方法 CT引导下穿刺接种法建立兔VX-2肝癌模型27只，行CT、超声检查观察肿瘤生长情况。2周后将实验动物随机分成空白对照组（注入生理盐水）、门静脉注射GNRs@SiO2-FA组和瘤内注射GNRs@SiO2-FA组，术后24、48、72 h每组各处死实验兔3只，取其肿瘤组织和各主要脏器并作冷冻切片，应用激光共聚焦显微镜观察实验动物对GNRs@SiO2-FA的摄取情况。结果成功构建兔VX-2肝癌模型，CT、超声检查显示肿瘤血供较丰富。共聚焦显微镜观察到实验动物摄入GNRs@SiO2-FA 24 h内即可特异性地与肿瘤细胞结合进入肿瘤细胞，并聚集在肿瘤细胞胞质内。结论 GNRs@SiO2-FA可在实验动物体内高度靶向性作用于肝癌细胞，在肿瘤靶向定位热疗和125I粒子介入治疗方面具有重要的应用前景。
목적건립토간암모형，관찰실험동물대협산（ FA ）우련이양화규포복적금납미봉（GNRs@SiO2-FA）적섭취상황，검측GNRs@SiO2-FA대간암세포적파향성。방법 CT인도하천자접충법건립토VX-2간암모형27지，행CT、초성검사관찰종류생장정황。2주후장실험동물수궤분성공백대조조（주입생리염수）、문정맥주사GNRs@SiO2-FA조화류내주사GNRs@SiO2-FA조，술후24、48、72 h매조각처사실험토3지，취기종류조직화각주요장기병작냉동절편，응용격광공취초현미경관찰실험동물대GNRs@SiO2-FA적섭취정황。결과성공구건토VX-2간암모형，CT、초성검사현시종류혈공교봉부。공취초현미경관찰도실험동물섭입GNRs@SiO2-FA 24 h내즉가특이성지여종류세포결합진입종류세포，병취집재종류세포포질내。결론 GNRs@SiO2-FA가재실험동물체내고도파향성작용우간암세포，재종류파향정위열료화125I입자개입치료방면구유중요적응용전경。
Objective To establish the rabbit model with hepatic VX-2 tumor and to investigate the intake of folate-conjugated silica-coated gold nanorods (GNRs@SiO2-FA) in experimental rabbits. Methods Under CT-guidance, animal model with VX-2 liver cancer was established in 27 rabbits by using puncture inoculation method. CT scanning and sonography were employed to observe the tumor growth. After two weeks, the rabbits were randomly and equally divided into blank control group (n=9, injection of saline), portal vein injection group (n=9, injection of GNRs@SiO2-FA) and intra-tumoral injection group (n=9, injection of GNRs@SiO2-FA). Every three rabbits from each group were sacrificed each time at 24 h, 48 h and 72 h after the treatment. The tumor tissue and the major organs were collected and sent for pathological examination. The cellular uptake of GNRs@SiO2-FA was studied by confocal laser scanning microscopy. Results The rabbit model of VX-2 liver cancer was successfully established. CT and sonography examination indicated that the tumor was rich in blood supply. Confocal laser scanning microscopy revealed that GNRs@SiO2-FA could specifically bind with tumor cells within 24 hours after injection, then the GNRs@SiO2-FA entered into the tumor cells and gathered in the tumor cytoplasm. Conclusion GNRs@SiO2-FA has highly targeted effect on the liver cancer cells in experimental animals, which has very important application prospect in targeting hyperthermia therapy and in 125I seed implantation therapy.