药学与临床研究
藥學與臨床研究
약학여림상연구
PHARMACEUTICAL AND CLINICAL RESEARCH
2015年
2期
104-106
,共3页
徐炳国%王树卿%刘程伟%高玲娟
徐炳國%王樹卿%劉程偉%高玲娟
서병국%왕수경%류정위%고령연
替吉奥单药%gC1qR基因%幽门螺杆菌(H. pylori,Hp)%胃癌
替吉奧單藥%gC1qR基因%幽門螺桿菌(H. pylori,Hp)%胃癌
체길오단약%gC1qR기인%유문라간균(H. pylori,Hp)%위암
Compound Tegafur and Oteracil Potassium Sustained Capsules (S-1)%gC1qR gene%H. pylori%Gastric cancer
目的:探讨替吉奥单药对胃癌中幽门螺杆菌(H. pylori,Hp)的感染及球状C1q受体基因(gC1qR)表达的影响。方法:以胃癌、胃炎病例组织及胃癌细胞系为研究对象,采用实时定量聚合酶链反应(Real_time_PCR)检测gC1qR_mRNA表达水平;采用Hp DNA_PCR方法,对胃窦部组织标本进行Hp水平检测;流式细胞技术进行细胞凋亡的检测及transwell实验进行细胞侵袭能力的检测。结果:在40对配对组织中有33对肿瘤组织gC1qR_mRNA表达低于配对的胃炎病例组织;替吉奥单药可诱导gC1qR基因表达,抑制胃癌细胞Hp感染,同时增加胃癌细胞的凋亡率及降低胃癌细胞侵袭能力。结论:替吉奥单药诱导gC1qR基因表达,影响Hp对胃癌发生发展,是治疗胃癌的首选药物。
目的:探討替吉奧單藥對胃癌中幽門螺桿菌(H. pylori,Hp)的感染及毬狀C1q受體基因(gC1qR)錶達的影響。方法:以胃癌、胃炎病例組織及胃癌細胞繫為研究對象,採用實時定量聚閤酶鏈反應(Real_time_PCR)檢測gC1qR_mRNA錶達水平;採用Hp DNA_PCR方法,對胃竇部組織標本進行Hp水平檢測;流式細胞技術進行細胞凋亡的檢測及transwell實驗進行細胞侵襲能力的檢測。結果:在40對配對組織中有33對腫瘤組織gC1qR_mRNA錶達低于配對的胃炎病例組織;替吉奧單藥可誘導gC1qR基因錶達,抑製胃癌細胞Hp感染,同時增加胃癌細胞的凋亡率及降低胃癌細胞侵襲能力。結論:替吉奧單藥誘導gC1qR基因錶達,影響Hp對胃癌髮生髮展,是治療胃癌的首選藥物。
목적:탐토체길오단약대위암중유문라간균(H. pylori,Hp)적감염급구상C1q수체기인(gC1qR)표체적영향。방법:이위암、위염병례조직급위암세포계위연구대상,채용실시정량취합매련반응(Real_time_PCR)검측gC1qR_mRNA표체수평;채용Hp DNA_PCR방법,대위두부조직표본진행Hp수평검측;류식세포기술진행세포조망적검측급transwell실험진행세포침습능력적검측。결과:재40대배대조직중유33대종류조직gC1qR_mRNA표체저우배대적위염병례조직;체길오단약가유도gC1qR기인표체,억제위암세포Hp감염,동시증가위암세포적조망솔급강저위암세포침습능력。결론:체길오단약유도gC1qR기인표체,영향Hp대위암발생발전,시치료위암적수선약물。
Objective: To investigate the effect of compound tegafur and oteracil potassium sustained capsules (S-1) on the expression of gC1qR gene and H. pylori (Hp) in gastric cancer. Methods: The expression of gC1qR mRNA and Hp was detected by Real time PCR and Hp DNA PCR in 40 matched pairs of gastritis and gastric cancer specimens. In vitro experiment, the apoptosis of gastric cancer cell line-treated with S-1 was assessed by flow cytometry, meanwhile, the migration of gastric cancer cell was detected via transwell assay. Results: The levels of gC1qR mRNA were significantly decreased and Hp was significantly increased in gastric cancer specimens. S-1 could induce the overexpression of gC1qR and inhibit the infection of Hp, and induce the apoptosis and inhibit the migration of gastric cancer cell. Conclusions: These results indicate that S -1 induces the expression of gC1qR, and effects the development of Hp-infected gastric cancer, S-1 is the first selective drug for controlling gastric cancer.