CAJ | 학술논문

目的：了解临床分离革兰阴性菌产 IMP 及VIM 金属β-内酰胺酶（MβLs）基因的检出情况，以及对β-内酰胺类抗生素的耐药状况。方法采用 K-B 法对临床分离的113株细菌进行药物敏感试验，采用聚合酶链反应（PCR）检测耐药基因 IMP 和 VIM ，测序并进行 BLAST 比对分析。结果1株荧光假单胞菌检出 VIM 基因；15株菌中检出 IMP 基因，其中肺炎克雷伯菌6株，鲍曼不动杆菌 3株，大肠埃希菌2株，罗尔斯顿菌、铜绿假单胞菌、无丙二酸柠檬酸杆菌、阴沟肠杆菌各1株。BLAST 结果显示，VIM 基因为VIM-2型，与基因库中序列相似度99％；IMP 基因均为 IMP-1亚型，相似度在98％～99％，高度同源。IMP 阳性菌株对头孢曲松、头孢噻肟、头孢西丁、氨曲南和亚胺培南的耐药率高于阴性菌株，差异均具有统计学意义（均 P ＜0．05）。结论不同菌株 IMP 基因高度同源，均为 IMP-1型，表明 IMP 基因的传播能力很强，可以突破种属的限制在不同细菌中传播。IMP 基因与细菌对β-内酰胺类抗生素耐药相关。
목적：료해림상분리혁란음성균산 IMP 급VIM 금속β-내선알매（MβLs）기인적검출정황，이급대β-내선알류항생소적내약상황。방법채용 K-B 법대림상분리적113주세균진행약물민감시험，채용취합매련반응（PCR）검측내약기인 IMP 화 VIM ，측서병진행 BLAST 비대분석。결과1주형광가단포균검출 VIM 기인；15주균중검출 IMP 기인，기중폐염극뢰백균6주，포만불동간균 3주，대장애희균2주，라이사돈균、동록가단포균、무병이산저몽산간균、음구장간균각1주。BLAST 결과현시，VIM 기인위VIM-2형，여기인고중서렬상사도99％；IMP 기인균위 IMP-1아형，상사도재98％～99％，고도동원。IMP 양성균주대두포곡송、두포새우、두포서정、안곡남화아알배남적내약솔고우음성균주，차이균구유통계학의의（균 P ＜0．05）。결론불동균주 IMP 기인고도동원，균위 IMP-1형，표명 IMP 기인적전파능력흔강，가이돌파충속적한제재불동세균중전파。IMP 기인여세균대β-내선알류항생소내약상관。
Objective To investigate the detection of IMP andVIM metallo-β-lactamases (MβLs)genes in clinically iso-lated gram-negative bacteria as well as bacterial resistance toβ-lactam antimicrobial agents.Methods 113 clinically isolated bacteria were performed antimicrobial susceptibility testing by Kirby-Bauer method ,drug-resistant genes IMP and VIM were detected by polymerase chain reaction (PCR),PCR products were sequenced and aligned with BLAST software. Results VIM gene was detected in 1 Pseudomonas fluorescens strain ,IMP gene was detected in 15 strains ,they were Klebsiella pneumoniae (n=6),Acinetobacter baumannii (n=3),Escherichia coli (n=2),Ralstonia picket-tii (n=1),Pseudomonas aeruginosa (n=1 ),Citrobacter amalonaticua (n=1 ),and Enterobacter cloacae (n=1 ). BLAST results showed that VIM gene was VIM-2 subtype,similarity with gene bank was 99%;all IMP genes were IMP-1 subtype,which were highly homologous ,similarity was 98%-99%.Resistant rates of IMP positive strains to ceftriaxone,cefotaxime,cefoxitin,aztreonam and imipenem were all significantly higher than negative strains (all P <0.05).Conclusion IMP genes of different strains are highly homologous,all are IMP-1 type,indi-cating that IMP genes are highly transmissible and can spread among different species of bacteria.IMP genes are related with resistance ofβ-lactam antimicrobial agents.