生物学杂志
生物學雜誌
생물학잡지
JOURNAL OF BIOLOGY
2015年
2期
42-47
,共6页
紫云英%硒胁迫%Hormesis效应%生理特性
紫雲英%硒脅迫%Hormesis效應%生理特性
자운영%서협박%Hormesis효응%생리특성
Astragalus sinicus%selenium stress%hormesis effect%physiological characteristic
采用水培方法，研究硒（ Se）不同浓度（0、0.2、0.5、1.0、1.5、2.0、2.5和5.0 mg／L）胁迫下紫云英的生长特征，探讨低浓度Se对紫云英的Hormesis效应，评价Se胁迫下紫云英的生理响应。结果表明，低浓度Se （≤0.5 mg／L）对紫云英根系相对伸长率、耐性指数、根系活力、叶绿素a含量、叶绿素b含量和类胡萝卜素含量均有显著的刺激效应，分别比对照增加了68.26％，9.00％，38.10％，21.6％，24.3％和66.7％；对其叶片蒸腾速率和叶片含水量也表现出一定的促进作用；Se浓度≥1.0 mg／L对紫云英的生长有明显的抑制作用。 Se浓度为5.0 mg／L时，紫云英地上部Se含量达到（73.03±1.151）μg／g DW，地下部Se 含量达到（48.427±1.525）μg／g DW。 Se对紫云英产生Hormesis效应的剂量范围为0.2～0.5 mg／L。结果还表明，根系MDA含量、根系相对电导率、地下部分生物量、根系耐性指数、根系活力、根系相对伸长率和地上部生物量这7项指标在评价紫云英幼苗耐Se性方面比较重要。
採用水培方法，研究硒（ Se）不同濃度（0、0.2、0.5、1.0、1.5、2.0、2.5和5.0 mg／L）脅迫下紫雲英的生長特徵，探討低濃度Se對紫雲英的Hormesis效應，評價Se脅迫下紫雲英的生理響應。結果錶明，低濃度Se （≤0.5 mg／L）對紫雲英根繫相對伸長率、耐性指數、根繫活力、葉綠素a含量、葉綠素b含量和類鬍蘿蔔素含量均有顯著的刺激效應，分彆比對照增加瞭68.26％，9.00％，38.10％，21.6％，24.3％和66.7％；對其葉片蒸騰速率和葉片含水量也錶現齣一定的促進作用；Se濃度≥1.0 mg／L對紫雲英的生長有明顯的抑製作用。 Se濃度為5.0 mg／L時，紫雲英地上部Se含量達到（73.03±1.151）μg／g DW，地下部Se 含量達到（48.427±1.525）μg／g DW。 Se對紫雲英產生Hormesis效應的劑量範圍為0.2～0.5 mg／L。結果還錶明，根繫MDA含量、根繫相對電導率、地下部分生物量、根繫耐性指數、根繫活力、根繫相對伸長率和地上部生物量這7項指標在評價紫雲英幼苗耐Se性方麵比較重要。
채용수배방법，연구서（ Se）불동농도（0、0.2、0.5、1.0、1.5、2.0、2.5화5.0 mg／L）협박하자운영적생장특정，탐토저농도Se대자운영적Hormesis효응，평개Se협박하자운영적생리향응。결과표명，저농도Se （≤0.5 mg／L）대자운영근계상대신장솔、내성지수、근계활력、협록소a함량、협록소b함량화류호라복소함량균유현저적자격효응，분별비대조증가료68.26％，9.00％，38.10％，21.6％，24.3％화66.7％；대기협편증등속솔화협편함수량야표현출일정적촉진작용；Se농도≥1.0 mg／L대자운영적생장유명현적억제작용。 Se농도위5.0 mg／L시，자운영지상부Se함량체도（73.03±1.151）μg／g DW，지하부Se 함량체도（48.427±1.525）μg／g DW。 Se대자운영산생Hormesis효응적제량범위위0.2～0.5 mg／L。결과환표명，근계MDA함량、근계상대전도솔、지하부분생물량、근계내성지수、근계활력、근계상대신장솔화지상부생물량저7항지표재평개자운영유묘내Se성방면비교중요。
A hydroponic experiment was conducted to investigate the growth characteristics of Astragalus sinicus under selenium ( Se) stress (0, 0.2, 0.5, 1.0, 1.5, 2.0, 2.5 and 5.0 mg/L), to investigate the Hormesis effect of low concentration Se on A.sinicus, and to evaluate the physiological response of A.sinicus to Se stress.The results showed that at low concentration of Se (≤0.5 mg/L) treatments, relative root elongation, root tolerance index, root activity, chlorophyll a contents, chlorophyll b contents, and carotenoid contents of A.sinicus increased significantly by 68.26%, 9.00%, 38.10%%, 21.6%, 24.3%, and 66.7%, respectively , as com-pared with that of the control .In addition, Se (≤0.5 mg/L) treatments also had a certain role in promoting transpiration rate and rel-ative water content of leaf .However, when the concentration of Se was greater than or equal to 1.0 mg/L, the growth of A.sinicus was inhibited significantly .When the Se concentration was 5.0 mg/L, the Se contents in the shoot and root of A.sinicus reached to (73.03 ±1.151)μg/g DW and (73.03 ±1.151)μg/g DW, respectively.The dose range of the hormesis effect of Se on A.sinicus was 0.2-0.5 mg/L.The result also showed that MDA content , relative conductivity , root biomass , root tolerance index , root activity , relative root elongation , and shoot biomass were important indicators in Se stress evaluation of A.sinicus seedlings .
